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Literature summary extracted from

  • Hong, J.S.; Park, S.J.; Parajuli, N.; Park, S.R.; Koh, H.S.; Jung, W.S.; Choi, C.Y.; Yoon, Y.J.
    Functional analysis of desVIII homologues involved in glycosylation of macrolide antibiotics by interspecies complementation (2006), Gene, 386, 123-130.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide Streptomyces venezuelae
-
 dTDP + 10-deoxymethymycin
-
 ?
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide Streptomyces venezuelae ATCC 15439
-
 dTDP + 10-deoxymethymycin
-
 ?
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide Streptomyces venezuelae
-
 dTDP + narbomycin
-
 ?
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide Streptomyces venezuelae ATCC 15439
-
 dTDP + narbomycin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.277 Streptomyces venezuelae Q9ZGH7
-
-
2.4.1.277 Streptomyces venezuelae ATCC 15439 Q9ZGH7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide
-
 Streptomyces venezuelae dTDP + 10-deoxymethymycin
-
 ?
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 10-deoxymethynolide
-
 Streptomyces venezuelae ATCC 15439 dTDP + 10-deoxymethymycin
-
 ?
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide
-
 Streptomyces venezuelae dTDP + narbomycin
-
 ?
2.4.1.277 TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide
-
 Streptomyces venezuelae ATCC 15439 dTDP + narbomycin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.277 macrolide glycosyltransferase DesVII
-
 Streptomyces venezuelae

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.277 DesVIII protein DesVIII is an auxiliary protein which enhances the transfer of TDP-D-desosamine catalyzed by DesVII glycosyltransferase in the biosynthesis of macrolide antibiotics, neomethymycin, methymycin and pikromycin, in Streptomyces venezuelae ATCC 15439. The DesVIII homologues (except for TylMIII) can functionally replace the native DesVIII for glycosylation to proceed in vivo and their functions are similar in acting as glycosyltransferase auxiliary proteins. Complementation by expressing EryCII, OleP1, and DnrQ in a desVIII deletion mutant strain restors the production of glycosylated macrolides to an approximate level of 66%, 26% and 26%, respectively, compared to self-complementation by DesVIII Streptomyces venezuelae