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Literature summary extracted from
- Human prostaglandin H synthase (hPHS)-1- and hPHS-2-dependent bioactivation, oxidative macromolecular damage, and cytotoxicity of dopamine, its precursor, and its metabolites (2011), Free Radic. Biol. Med., 50, 295-304.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | arachidonic acid | exogenous, increases hPHS activity | Homo sapiens |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | expression of isozymes PHS-1 and PHS-2 in CHO-K1 cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | DUP-697 | a standard PHS-2 inhibitor | Homo sapiens | |
| 1.14.99.1 | SC-560 | a standard PHS-1 inhibitor | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | arachidonate + AH2 + O2 | Homo sapiens | - |
prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | additional information | Homo sapiens | dopamine precursor L-dihydroxyphenylalanine, i.e. L-DOPA, and metabolites dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine may serve as substrates for prostaglandin H synthase-catalyzed bioactivation to free radical intermediates | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | arachidonate + AH2 + O2 | - |
Homo sapiens | prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | additional information | dopamine precursor L-dihydroxyphenylalanine, i.e. L-DOPA, and metabolites dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine may serve as substrates for prostaglandin H synthase-catalyzed bioactivation to free radical intermediates | Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.1 | PHS-1 | - |
Homo sapiens |
| 1.14.99.1 | PHS-2 | - |
Homo sapiens |
| 1.14.99.1 | prostaglandin H synthase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 7.4 | - |
assay at | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.99.1 | physiological function | PHS isozyme-dependent oxidative damage to proteins and DNA, and cytotoxicity, overview. hPHS-1- and hPHS-2-expressing cells incubated with dopamine, L-dihydroxyphenylalanine, dihydroxyphenylacetic acid, or homovanillic acid exhibit increased cytotoxicity compared to untransfected cells, and cytotoxicity is increased further by exogenous arachidonic acid, which increases hPHS activity. Isozyme-specific, PHS-dependent oxidative damage and cytotoxicity caused by neurotransmitters, their precursors, and their metabolites may contribute to neurodegeneration associated with aging | Homo sapiens |
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