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Literature summary extracted from

  • Topakas, E.; Kyriakopoulos, S.; Biely, P.; Hirsch, J.; Vafiadi, C.; Christakopoulos, P.
    Carbohydrate esterases of family 2 are 6-O-deacetylases (2010), FEBS Lett., 584, 543-548.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.6 expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus
3.1.1.6 expressed in Escherichia coli BL21(DE3) cells Cellvibrio japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.6 1.24
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 1.55
-
 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 2.46
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Acetivibrio thermocellus
3.1.1.6 4.31
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 4.42
-
 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Acetivibrio thermocellus
-
-
-
3.1.1.6 Cellvibrio japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 immobilized metal affinity chromatography Acetivibrio thermocellus
3.1.1.6 immobilized metal affinity chromatography Cellvibrio japonicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.6 2
-
 isozyme CE2, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C Acetivibrio thermocellus
3.1.1.6 2
-
 isozyme CE2C, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 3
-
 isozyme CE2C, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 3
-
 isozyme CE2C, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 4
-
 isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 5
-
 isozyme CE2B, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 8
-
 isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C Acetivibrio thermocellus
3.1.1.6 35
-
 isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C Acetivibrio thermocellus
3.1.1.6 60
-
 isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 183
-
 isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C Acetivibrio thermocellus
3.1.1.6 183
-
 isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 328
-
 isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C Cellvibrio japonicus
3.1.1.6 395
-
 isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C Cellvibrio japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 3-O-methyl-D-glucopyranoside + vinyl acetate
-
 Acetivibrio thermocellus 6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
 ?
3.1.1.6 3-O-methyl-D-glucopyranoside + vinyl acetate
-
 Cellvibrio japonicus 6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
 ?
3.1.1.6 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 6-O-acetyl-D-mannopyranose + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 6-O-acetyl-D-mannopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 alpha,alpha-trehalose + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 cellobiose + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 cellobiose + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 D-galactose + vinyl acetate isozyme CE2B shows 22% transacetylation conversion, isozyme CE2A shows 48% transacetylation conversion Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 D-galactose + vinyl acetate isozyme CE2B shows 89% transacetylation conversion Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 D-glucose + vinyl acetate isozyme CE2B shows 31% transacetylation conversion, isozyme CE2A shows 25% transacetylation conversion Cellvibrio japonicus 6-O-acetyl-D-glucopyranose + vinyl alcohol
-
 ?
3.1.1.6 D-glucose + vinyl acetate isozyme CE2B shows 86% transacetylation conversion Acetivibrio thermocellus 6-O-acetyl-D-glucopyranose + vinyl alcohol
-
 ?
3.1.1.6 D-mannose + vinyl acetate isozyme CE2B shows 46% transacetylation conversion, isozyme CE2A shows 26% transacetylation conversion Cellvibrio japonicus 6-O-acetyl-D-mannopyranose + vinyl alcohol
-
 ?
3.1.1.6 D-mannose + vinyl acetate isozyme CE2B shows 71% transacetylation conversion Acetivibrio thermocellus 6-O-acetyl-D-mannopyranose + vinyl alcohol
-
 ?
3.1.1.6 D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.1.1.6 methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
-
 Cellvibrio japonicus ?
-
 ?
3.1.1.6 methyl alpha-D-glucopyranoside + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl alpha-D-glucopyranoside + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl beta-D-glucopyranoside + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 methyl beta-D-glucopyranoside + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?
3.1.1.6 additional information no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside Acetivibrio thermocellus ?
-
 ?
3.1.1.6 additional information no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside Cellvibrio japonicus ?
-
 ?
3.1.1.6 additional information the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors Acetivibrio thermocellus ?
-
 ?
3.1.1.6 additional information the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors Cellvibrio japonicus ?
-
 ?
3.1.1.6 xylobiose + vinyl acetate
-
 Acetivibrio thermocellus ? + vinyl alcohol
-
 ?
3.1.1.6 xylobiose + vinyl acetate
-
 Cellvibrio japonicus ? + vinyl alcohol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 6-O-deacetylase
-
 Acetivibrio thermocellus
3.1.1.6 6-O-deacetylase
-
 Cellvibrio japonicus
3.1.1.6 ACE
-
 Acetivibrio thermocellus
3.1.1.6 ACE
-
 Cellvibrio japonicus
3.1.1.6 Acetyl esterase
-
 Acetivibrio thermocellus
3.1.1.6 Acetyl esterase
-
 Cellvibrio japonicus
3.1.1.6 CE2
-
 Acetivibrio thermocellus
3.1.1.6 CE2A isozyme Cellvibrio japonicus
3.1.1.6 CE2B isozyme Cellvibrio japonicus
3.1.1.6 CE2C isozyme Cellvibrio japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.6 41.6
-
 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 241.6
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 1331
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Acetivibrio thermocellus
3.1.1.6 2101
-
 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 3210
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.6 9.42
-
 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 56.08
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 541.5
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Acetivibrio thermocellus
3.1.1.6 1356
-
 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus
3.1.1.6 2599
-
 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C Cellvibrio japonicus