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Literature summary extracted from
- Identification of conserved histidines and glutamic acid as key residues for isomerohydrolase activity of RPE65, an enzyme of the visual cycle in the retinal pigment epithelium (2005), FEBS Lett., 579, 5414-5418.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.64 | expressed in 293A-LRAT cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.64 | H180A | the mutant shows about 20% decreased protein levels and abolished enzymatic activity | Homo sapiens |
| 3.1.1.64 | H241A | the mutant shows about 80% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide | Homo sapiens |
| 3.1.1.64 | H313A | the mutant shows about 10% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide | Homo sapiens |
| 3.1.1.64 | H527A | the mutant shows about 20% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide | Homo sapiens |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.64 | wild type RPE65 has a half-life of more than 10 h after the addition of cycloheximide | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.64 | Homo sapiens | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.64 | retinal pigment epithelium | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.64 | all-trans-retinyl ester + H2O | - |
Homo sapiens | 11-cis-retinol + a fatty acid | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.64 | Rpe65 | - |
Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.64 | physiological function | RPE65 from the retinal pigment epithelium is a critical enzyme in the visual cycle for regeneration of 11-cis retinal | Homo sapiens |
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Release 2023.1 (January 2023)
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