BRENDA - Enzyme Database show

Identification of conserved histidines and glutamic acid as key residues for isomerohydrolase activity of RPE65, an enzyme of the visual cycle in the retinal pigment epithelium

Takahashi, Y.; Moiseyev, G.; Chen, Y.; Ma, J.X.; FEBS Lett. 579, 5414-5418 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-LRAT cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.64
H180A
the mutant shows about 20% decreased protein levels and abolished enzymatic activity
Homo sapiens
3.1.1.64
H241A
the mutant shows about 80% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide
Homo sapiens
3.1.1.64
H313A
the mutant shows about 10% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide
Homo sapiens
3.1.1.64
H527A
the mutant shows about 20% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide
Homo sapiens
General Stability
EC Number
General Stability
Organism
3.1.1.64
wild type RPE65 has a half-life of more than 10 h after the addition of cycloheximide
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
715009
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-LRAT cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.64
H180A
the mutant shows about 20% decreased protein levels and abolished enzymatic activity
Homo sapiens
3.1.1.64
H241A
the mutant shows about 80% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide
Homo sapiens
3.1.1.64
H313A
the mutant shows about 10% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide
Homo sapiens
3.1.1.64
H527A
the mutant shows about 20% decreased protein levels and abolished enzymatic activity and significantly decreased protein stabilities in the presence of cycloheximide
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.64
wild type RPE65 has a half-life of more than 10 h after the addition of cycloheximide
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
715009
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
RPE65 from the retinal pigment epithelium is a critical enzyme in the visual cycle for regeneration of 11-cis retinal
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
RPE65 from the retinal pigment epithelium is a critical enzyme in the visual cycle for regeneration of 11-cis retinal
Homo sapiens