EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.64 | expressed in 293A-lecithin retinol acyltransferase cells | Danio rerio |
3.1.1.90 | expressed in 293A-LRAT and 293T | Danio rerio |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.64 | bipyridine | the addition of 6 mM FeSO4 into the iron chelator reaction restores partial enzyme activity | Danio rerio | |
3.1.1.90 | bipyridine | complete inhibition at 1 mM | Danio rerio |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.64 | 0.0026 | - |
an all-trans retinyl ester | pH and temperature not specified in the publication | Danio rerio | |
3.1.1.90 | 0.0026 | - |
all-trans retinyl ester | pH and temperature not specified in the publication | Danio rerio |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.64 | cytosol | - |
Danio rerio | 5829 | - |
3.1.1.64 | membrane | - |
Danio rerio | 16020 | - |
3.1.1.90 | membrane | - |
Danio rerio | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.64 | Fe2+ | enzymatic activity is dependent on iron | Danio rerio | |
3.1.1.90 | Fe2+ | dependent on | Danio rerio |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.64 | 61000 | - |
x * 61000, estimated from SDS-PAGE | Danio rerio |
3.1.1.90 | 61000 | - |
x * 61000, recombinant enzyme, SDS-PAGE | Danio rerio |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.64 | Danio rerio | A9C3R9 | - |
- |
3.1.1.90 | Danio rerio | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.64 | Ni-NTA resin column chromatography | Danio rerio |
3.1.1.90 | Ni-NTA column chromatography | Danio rerio |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.64 | brain | 13cIMH expression is detected in the periventricular grey zone of the optic tectum and torus longitudinalis, at the fasciculus longitudinalis medialis in the medulla oblongata, and at the periventricular pretectum | Danio rerio | - |
3.1.1.90 | brain | high expression | Danio rerio | - |
3.1.1.90 | eye | low expression | Danio rerio | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.64 | an all-trans retinyl ester + H2O | specific substrate | Danio rerio | 13-cis-retinol + a fatty acid | - |
? | |
3.1.1.90 | all-trans retinyl ester + H2O | - |
Danio rerio | 13-cis retinol + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.64 | ? | x * 61000, estimated from SDS-PAGE | Danio rerio |
3.1.1.90 | ? | x * 61000, recombinant enzyme, SDS-PAGE | Danio rerio |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.64 | 13-cis isomerohydrolase | - |
Danio rerio |
3.1.1.64 | 13cIMH | - |
Danio rerio |
3.1.1.90 | 13-cis isomerohydrolase | - |
Danio rerio |
3.1.1.90 | 13-cis retinoid-specific isomerohydrolase | - |
Danio rerio |
3.1.1.90 | 13cIMH | - |
Danio rerio |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.64 | 0.00044 | - |
an all-trans retinyl ester | pH and temperature not specified in the publication | Danio rerio | |
3.1.1.90 | 0.00044 | - |
all-trans retinyl ester | pH and temperature not specified in the publication | Danio rerio |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.1.64 | physiological function | 13cIMH may play a key role in the modulation of neuronal functions in the brain | Danio rerio |
3.1.1.90 | physiological function | the enzyme plays a key role in the generation of 13-cis retinoic acid, as well as in the modulation of neuronal functions in the brain | Danio rerio |