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Literature summary extracted from

  • Takahashi, Y.; Moiseyev, G.; Chen, Y.; Farjo, K.; Nikolaeva, O.; Ma, J.X.
    An enzymatic mechanism for generating the precursor of endogenous 13-cis retinoic acid in the brain (2011), FEBS J., 278, 973-987.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.64 expressed in 293A-lecithin retinol acyltransferase cells Danio rerio
3.1.1.90 expressed in 293A-LRAT and 293T Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.64 bipyridine the addition of 6 mM FeSO4 into the iron chelator reaction restores partial enzyme activity Danio rerio
3.1.1.90 bipyridine complete inhibition at 1 mM Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.64 0.0026
-
an all-trans retinyl ester pH and temperature not specified in the publication Danio rerio
3.1.1.90 0.0026
-
all-trans retinyl ester pH and temperature not specified in the publication Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.64 cytosol
-
Danio rerio 5829
-
3.1.1.64 membrane
-
Danio rerio 16020
-
3.1.1.90 membrane
-
Danio rerio 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.64 Fe2+ enzymatic activity is dependent on iron Danio rerio
3.1.1.90 Fe2+ dependent on Danio rerio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.64 61000
-
x * 61000, estimated from SDS-PAGE Danio rerio
3.1.1.90 61000
-
x * 61000, recombinant enzyme, SDS-PAGE Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.64 Danio rerio A9C3R9
-
-
3.1.1.90 Danio rerio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.64 Ni-NTA resin column chromatography Danio rerio
3.1.1.90 Ni-NTA column chromatography Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.64 brain 13cIMH expression is detected in the periventricular grey zone of the optic tectum and torus longitudinalis, at the fasciculus longitudinalis medialis in the medulla oblongata, and at the periventricular pretectum Danio rerio
-
3.1.1.90 brain high expression Danio rerio
-
3.1.1.90 eye low expression Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.64 an all-trans retinyl ester + H2O specific substrate Danio rerio 13-cis-retinol + a fatty acid
-
?
3.1.1.90 all-trans retinyl ester + H2O
-
Danio rerio 13-cis retinol + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.64 ? x * 61000, estimated from SDS-PAGE Danio rerio
3.1.1.90 ? x * 61000, recombinant enzyme, SDS-PAGE Danio rerio

Synonyms

EC Number Synonyms Comment Organism
3.1.1.64 13-cis isomerohydrolase
-
Danio rerio
3.1.1.64 13cIMH
-
Danio rerio
3.1.1.90 13-cis isomerohydrolase
-
Danio rerio
3.1.1.90 13-cis retinoid-specific isomerohydrolase
-
Danio rerio
3.1.1.90 13cIMH
-
Danio rerio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.64 0.00044
-
an all-trans retinyl ester pH and temperature not specified in the publication Danio rerio
3.1.1.90 0.00044
-
all-trans retinyl ester pH and temperature not specified in the publication Danio rerio

General Information

EC Number General Information Comment Organism
3.1.1.64 physiological function 13cIMH may play a key role in the modulation of neuronal functions in the brain Danio rerio
3.1.1.90 physiological function the enzyme plays a key role in the generation of 13-cis retinoic acid, as well as in the modulation of neuronal functions in the brain Danio rerio