BRENDA - Enzyme Database show

An enzymatic mechanism for generating the precursor of endogenous 13-cis retinoic acid in the brain

Takahashi, Y.; Moiseyev, G.; Chen, Y.; Farjo, K.; Nikolaeva, O.; Ma, J.X.; FEBS J. 278, 973-987 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-lecithin retinol acyltransferase cells
Danio rerio
3.1.1.90
expressed in 293A-LRAT and 293T
Danio rerio
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
bipyridine
the addition of 6 mM FeSO4 into the iron chelator reaction restores partial enzyme activity
Danio rerio
3.1.1.90
bipyridine
complete inhibition at 1 mM
Danio rerio
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.64
0.0026
-
an all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
3.1.1.90
0.0026
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
cytosol
-
Danio rerio
5829
-
3.1.1.64
membrane
-
Danio rerio
16020
-
3.1.1.90
membrane
-
Danio rerio
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
enzymatic activity is dependent on iron
Danio rerio
3.1.1.90
Fe2+
dependent on
Danio rerio
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.64
61000
-
x * 61000, estimated from SDS-PAGE
Danio rerio
3.1.1.90
61000
-
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Danio rerio
A9C3R9
-
-
3.1.1.90
Danio rerio
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.64
Ni-NTA resin column chromatography
Danio rerio
3.1.1.90
Ni-NTA column chromatography
Danio rerio
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
brain
13cIMH expression is detected in the periventricular grey zone of the optic tectum and torus longitudinalis, at the fasciculus longitudinalis medialis in the medulla oblongata, and at the periventricular pretectum
Danio rerio
-
3.1.1.90
brain
high expression
Danio rerio
-
3.1.1.90
eye
low expression
Danio rerio
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
an all-trans retinyl ester + H2O
specific substrate
714991
Danio rerio
13-cis-retinol + a fatty acid
-
-
-
?
3.1.1.90
all-trans retinyl ester + H2O
-
714991
Danio rerio
13-cis retinol + ?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.64
?
x * 61000, estimated from SDS-PAGE
Danio rerio
3.1.1.90
?
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.1.64
0.00044
-
an all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
3.1.1.90
0.00044
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-lecithin retinol acyltransferase cells
Danio rerio
3.1.1.90
expressed in 293A-LRAT and 293T
Danio rerio
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
bipyridine
the addition of 6 mM FeSO4 into the iron chelator reaction restores partial enzyme activity
Danio rerio
3.1.1.90
bipyridine
complete inhibition at 1 mM
Danio rerio
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.64
0.0026
-
an all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
3.1.1.90
0.0026
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
cytosol
-
Danio rerio
5829
-
3.1.1.64
membrane
-
Danio rerio
16020
-
3.1.1.90
membrane
-
Danio rerio
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
enzymatic activity is dependent on iron
Danio rerio
3.1.1.90
Fe2+
dependent on
Danio rerio
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.64
61000
-
x * 61000, estimated from SDS-PAGE
Danio rerio
3.1.1.90
61000
-
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
Ni-NTA resin column chromatography
Danio rerio
3.1.1.90
Ni-NTA column chromatography
Danio rerio
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
brain
13cIMH expression is detected in the periventricular grey zone of the optic tectum and torus longitudinalis, at the fasciculus longitudinalis medialis in the medulla oblongata, and at the periventricular pretectum
Danio rerio
-
3.1.1.90
brain
high expression
Danio rerio
-
3.1.1.90
eye
low expression
Danio rerio
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
an all-trans retinyl ester + H2O
specific substrate
714991
Danio rerio
13-cis-retinol + a fatty acid
-
-
-
?
3.1.1.90
all-trans retinyl ester + H2O
-
714991
Danio rerio
13-cis retinol + ?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.64
?
x * 61000, estimated from SDS-PAGE
Danio rerio
3.1.1.90
?
x * 61000, recombinant enzyme, SDS-PAGE
Danio rerio
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.1.64
0.00044
-
an all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
3.1.1.90
0.00044
-
all-trans retinyl ester
pH and temperature not specified in the publication
Danio rerio
General Information
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
13cIMH may play a key role in the modulation of neuronal functions in the brain
Danio rerio
3.1.1.90
physiological function
the enzyme plays a key role in the generation of 13-cis retinoic acid, as well as in the modulation of neuronal functions in the brain
Danio rerio
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
13cIMH may play a key role in the modulation of neuronal functions in the brain
Danio rerio
3.1.1.90
physiological function
the enzyme plays a key role in the generation of 13-cis retinoic acid, as well as in the modulation of neuronal functions in the brain
Danio rerio