BRENDA - Enzyme Database show

An alternative isomerohydrolase in the retinal Mueller cells of a cone-dominant species

Takahashi, Y.; Moiseyev, G.; Chen, Y.; Nikolaeva, O.; Ma, J.X.; FEBS J. 278, 2913-2926 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A cells; expressed in 293A cells
Danio rerio
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
bipyridine
in the presence of 1 mM of the metal chelator bipyridine the enzymatic activity of isoform RPE65c is almost completely abolished
Danio rerio
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
cytosol
;
Danio rerio
5829
-
3.1.1.64
membrane
;
Danio rerio
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
isoform RPE65a requires iron for its enzymatic activity; isoform RPE65c requires iron for its enzymatic activity
Danio rerio
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Danio rerio
A9C3R8
isoform RPE65c
-
3.1.1.64
Danio rerio
Q6PBW5
isoform RPE65a
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
additional information
isoform RPE65c is not detected in the retinal pigment epithelium
Danio rerio
-
3.1.1.64
Mueller cell
;
Danio rerio
-
3.1.1.64
retina
;
Danio rerio
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
714986
Danio rerio
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
all-trans-retinyl ester + H2O
intrinsic substrate for isoform RPE65c
714986
Danio rerio
11-cis-retinol + 13-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
additional information
isoform RPE65c does not generate any detectable 11-cis-retinol from all-trans-retinol
714986
Danio rerio
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A cells
Danio rerio
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
bipyridine
in the presence of 1 mM of the metal chelator bipyridine the enzymatic activity of isoform RPE65c is almost completely abolished
Danio rerio
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
cytosol
-
Danio rerio
5829
-
3.1.1.64
membrane
-
Danio rerio
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
isoform RPE65a requires iron for its enzymatic activity
Danio rerio
3.1.1.64
Fe2+
isoform RPE65c requires iron for its enzymatic activity
Danio rerio
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
additional information
isoform RPE65c is not detected in the retinal pigment epithelium
Danio rerio
-
3.1.1.64
Mueller cell
-
Danio rerio
-
3.1.1.64
retina
-
Danio rerio
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
714986
Danio rerio
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
all-trans-retinyl ester + H2O
intrinsic substrate for isoform RPE65c
714986
Danio rerio
11-cis-retinol + 13-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
additional information
isoform RPE65c does not generate any detectable 11-cis-retinol from all-trans-retinol
714986
Danio rerio
?
-
-
-
-
General Information
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
isoform RPE65c is the alternative isomerohydrolase in the intra-retinal visual cycle, providing 11-cis retinal to cone photoreceptors in cone-dominant species
Danio rerio
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
isoform RPE65c is the alternative isomerohydrolase in the intra-retinal visual cycle, providing 11-cis retinal to cone photoreceptors in cone-dominant species
Danio rerio