BRENDA - Enzyme Database

Glutamate racemization and catabolism in Fusobacterium varium

Ramezani, M.; Resmer, K.L.; White, R.L.; FEBS J. 278, 2540-2551 (2011)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.1
additional information
charcoal adsorbs coenzyme B12 and inactivates glutamate mutase
Fusobacterium varium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.99.1
Co2+
essential constituent of coenzyme B12
Fusobacterium varium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.1.3
20000
-
gel filtration
Fusobacterium varium
5.1.1.3
29000
-
calculated from amino acid sequence
Fusobacterium varium
5.4.99.1
15000
-
x * 15000 + x * 51000, about
Fusobacterium varium
5.4.99.1
51000
-
x * 15000 + x * 51000, about
Fusobacterium varium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
L-threo-3-methylaspartate
Fusobacterium varium
-
L-glutamate
-
-
?
5.4.99.1
L-threo-3-methylaspartate
Fusobacterium varium NCTC 10560 / ATCC 8501
-
L-glutamate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Fusobacterium varium
-
-
-
5.1.1.3
Fusobacterium varium
-
-
-
5.1.1.3
Fusobacterium varium NCTC 10560 / ATCC 8501
-
-
-
5.4.99.1
Fusobacterium varium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.1.1.3
ammonium sulfate precipitation
Fusobacterium varium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.1.3
D-glutamate
-
714983
Fusobacterium varium
L-glutamate
-
-
-
?
5.1.1.3
D-glutamate
-
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
L-glutamate
-
-
-
?
5.1.1.3
L-glutamate
-
714983
Fusobacterium varium
D-glutamate
-
-
-
?
5.1.1.3
L-glutamate
-
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
D-glutamate
-
-
-
?
5.4.99.1
L-threo-3-methylaspartate
-
714983
Fusobacterium varium
L-glutamate
-
-
-
?
5.4.99.1
L-threo-3-methylaspartate
equilibrium constants favouring glutamate over 3-methylaspartate, formation of L-threo-(2S,3S)- and L-erythro-(2S,3R)-3-methylaspartate in the reverse reaction direction
714983
Fusobacterium varium
L-glutamate
no activity with D-glutamate
-
-
?
5.4.99.1
L-threo-3-methylaspartate
-
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
L-glutamate
-
-
-
?
5.4.99.1
L-threo-3-methylaspartate
equilibrium constants favouring glutamate over 3-methylaspartate, formation of L-threo-(2S,3S)- and L-erythro-(2S,3R)-3-methylaspartate in the reverse reaction direction
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
L-glutamate
no activity with D-glutamate
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
x * 15000 + x * 51000, about
Fusobacterium varium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.1.1.3
additional information
the enzyme is cofactor-independent
Fusobacterium varium
5.4.99.1
5'-deoxyadenosylcobalamin
i.e. coenzyme B12, required for activity
Fusobacterium varium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.1.1.3
additional information
the enzyme is cofactor-independent
Fusobacterium varium
5.4.99.1
5'-deoxyadenosylcobalamin
i.e. coenzyme B12, required for activity
Fusobacterium varium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.1
additional information
charcoal adsorbs coenzyme B12 and inactivates glutamate mutase
Fusobacterium varium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.99.1
Co2+
essential constituent of coenzyme B12
Fusobacterium varium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.1.1.3
20000
-
gel filtration
Fusobacterium varium
5.1.1.3
29000
-
calculated from amino acid sequence
Fusobacterium varium
5.4.99.1
15000
-
x * 15000 + x * 51000, about
Fusobacterium varium
5.4.99.1
51000
-
x * 15000 + x * 51000, about
Fusobacterium varium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.99.1
L-threo-3-methylaspartate
Fusobacterium varium
-
L-glutamate
-
-
?
5.4.99.1
L-threo-3-methylaspartate
Fusobacterium varium NCTC 10560 / ATCC 8501
-
L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.1.3
ammonium sulfate precipitation
Fusobacterium varium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.1.3
D-glutamate
-
714983
Fusobacterium varium
L-glutamate
-
-
-
?
5.1.1.3
D-glutamate
-
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
L-glutamate
-
-
-
?
5.1.1.3
L-glutamate
-
714983
Fusobacterium varium
D-glutamate
-
-
-
?
5.1.1.3
L-glutamate
-
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
D-glutamate
-
-
-
?
5.4.99.1
L-threo-3-methylaspartate
-
714983
Fusobacterium varium
L-glutamate
-
-
-
?
5.4.99.1
L-threo-3-methylaspartate
equilibrium constants favouring glutamate over 3-methylaspartate, formation of L-threo-(2S,3S)- and L-erythro-(2S,3R)-3-methylaspartate in the reverse reaction direction
714983
Fusobacterium varium
L-glutamate
no activity with D-glutamate
-
-
?
5.4.99.1
L-threo-3-methylaspartate
-
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
L-glutamate
-
-
-
?
5.4.99.1
L-threo-3-methylaspartate
equilibrium constants favouring glutamate over 3-methylaspartate, formation of L-threo-(2S,3S)- and L-erythro-(2S,3R)-3-methylaspartate in the reverse reaction direction
714983
Fusobacterium varium NCTC 10560 / ATCC 8501
L-glutamate
no activity with D-glutamate
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.99.1
?
x * 15000 + x * 51000, about
Fusobacterium varium
Expression
EC Number
Organism
Commentary
Expression
5.1.1.3
Fusobacterium varium
the racemase is constitutive and not induced by D-glutamate
additional information
General Information
EC Number
General Information
Commentary
Organism
4.3.1.2
physiological function
major role for the methylaspartate pathway of glutamate catabolism in Fusobacterium varium and substantial participation of the hydroxyglutarate pathway when coenzyme B12 is not available
Fusobacterium varium
5.4.99.1
metabolism
glutamate mutase and methylaspartase are the first two enzymes in the methylaspartate pathway, overview
Fusobacterium varium
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.3.1.2
physiological function
major role for the methylaspartate pathway of glutamate catabolism in Fusobacterium varium and substantial participation of the hydroxyglutarate pathway when coenzyme B12 is not available
Fusobacterium varium
5.4.99.1
metabolism
glutamate mutase and methylaspartase are the first two enzymes in the methylaspartate pathway, overview
Fusobacterium varium
Expression (protein specific)
EC Number
Organism
Commentary
Expression
5.1.1.3
Fusobacterium varium
the racemase is constitutive and not induced by D-glutamate
additional information