BRENDA - Enzyme Database

Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis

Espariz, M.; Repizo, G.; Blancato, V.; Mortera, P.; Alarcon, S.; Magni, C.; FEBS J. 278, 2140-2151 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.1.112
expressed in Escherichia coli BL21(DE3) cells
Enterococcus faecalis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.112
ADP
78% residual activity at 0.25 mM
Enterococcus faecalis
4.1.1.112
ATP
37% residual activity at 1 mM
Enterococcus faecalis
4.1.1.112
citrate
74% residual activity at 2 mM
Enterococcus faecalis
4.1.1.112
EDTA
complete inhibition at 2 mM
Enterococcus faecalis
4.1.1.112
malate
65% residual activity at 2 mM
Enterococcus faecalis
4.1.1.112
malonate
26% residual activity at 2 mM
Enterococcus faecalis
4.1.1.112
additional information
not inhibited by succinate
Enterococcus faecalis
4.1.1.112
NAD+
40% residual activity at 0.25 mM
Enterococcus faecalis
4.1.1.112
NADH
53% residual activity at 0.25 mM
Enterococcus faecalis
4.1.1.112
oxalate
14% residual activity at 2 mM
Enterococcus faecalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.112
0.62
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.112
Mn2+
the enzyme is dependent on divalent metal ions with 100% activity at 20 mM Mn2+
Enterococcus faecalis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.112
Enterococcus faecalis
Q82YW6
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.112
Ni2+-bounded affinity column chromatography
Enterococcus faecalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.112
Oxaloacetate
-
714979
Enterococcus faecalis
Pyruvate + CO2
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.112
11.2
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.112
4.5
-
-
Enterococcus faecalis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.112
3.5
5
-
Enterococcus faecalis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.112
additional information
no nicotinamide cofactors required
Enterococcus faecalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.112
expressed in Escherichia coli BL21(DE3) cells
Enterococcus faecalis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.112
additional information
no nicotinamide cofactors required
Enterococcus faecalis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.112
ADP
78% residual activity at 0.25 mM
Enterococcus faecalis
4.1.1.112
ATP
37% residual activity at 1 mM
Enterococcus faecalis
4.1.1.112
citrate
74% residual activity at 2 mM
Enterococcus faecalis
4.1.1.112
EDTA
complete inhibition at 2 mM
Enterococcus faecalis
4.1.1.112
malate
65% residual activity at 2 mM
Enterococcus faecalis
4.1.1.112
malonate
26% residual activity at 2 mM
Enterococcus faecalis
4.1.1.112
additional information
not inhibited by succinate
Enterococcus faecalis
4.1.1.112
NAD+
40% residual activity at 0.25 mM
Enterococcus faecalis
4.1.1.112
NADH
53% residual activity at 0.25 mM
Enterococcus faecalis
4.1.1.112
oxalate
14% residual activity at 2 mM
Enterococcus faecalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.112
0.62
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.112
Mn2+
the enzyme is dependent on divalent metal ions with 100% activity at 20 mM Mn2+
Enterococcus faecalis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.112
Ni2+-bounded affinity column chromatography
Enterococcus faecalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.112
Oxaloacetate
-
714979
Enterococcus faecalis
Pyruvate + CO2
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.112
11.2
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.112
4.5
-
-
Enterococcus faecalis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.112
3.5
5
-
Enterococcus faecalis
General Information
EC Number
General Information
Commentary
Organism
4.1.1.112
malfunction
the citrate fermentation phenotype is not affected by citM deletion
Enterococcus faecalis
4.1.1.112
metabolism
CitM is not required for efficient citrate utilization
Enterococcus faecalis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.1.1.112
malfunction
the citrate fermentation phenotype is not affected by citM deletion
Enterococcus faecalis
4.1.1.112
metabolism
CitM is not required for efficient citrate utilization
Enterococcus faecalis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.1.1.112
22.3
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.1.1.112
22.3
-
oxaloacetate
calculated value at pH 4.5, temperature not specified in the publication
Enterococcus faecalis