Literature summary extracted from
Bujacz, A.; Jedrzejczak-Krzepkowska, M.; Bielecki, S.; Redzynia, I.; Bujacz, G.
Crystal structures of the apo form of beta-fructofuranosidase from Bifidobacterium longum and its complex with fructose (2011), FEBS J., 278, 1728-1744.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.26 |
expression in Escherichia coli |
Bifidobacterium longum |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.26 |
apo form of beta-fructofuranosidase and its complex with fructose, both to 1.8 A resolution. Enzyme consists of the N-terminal five-blade beta-propeller domain that includes the catalytic site, as well as the beta-sandwich C-terminal domain. The active site is located at the bottom of the axial funnel created by the interblade loops and the loops between beta-strand 2-3 of each blade. The protein has a 35-residue elongation of the N-terminus containing a fiveturn alpha-helix, which distinguishes it from the other known members of the GH32 family |
Bifidobacterium longum |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.26 |
59156 |
- |
x * 59156, calculated for recombinant protein. x * 60000, SDS-PAGE |
Bifidobacterium longum |
3.2.1.26 |
60000 |
- |
x * 59156, calculated for recombinant protein. x * 60000, SDS-PAGE |
Bifidobacterium longum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.26 |
Bifidobacterium longum |
A2TLS9 |
- |
- |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.26 |
? |
x * 59156, calculated for recombinant protein. x * 60000, SDS-PAGE |
Bifidobacterium longum |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
3.2.1.26 |
Bifidobacterium longum |
calculated |
- |
4.9 |