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Literature summary extracted from

  • de Sanctis, D.; Inacio, J.M.; Lindley, P.F.; de Sa-Nogueira, I.; Bento, I.
    New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases (2010), FEBS J., 277, 4562-4574.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 expressed in Escherichia coli BL21(DE3) pLysS cells Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.99 sitting drop vapor diffusion method, using 65% (w/v) 2-methyl-2,4-pentadiol and 100 mM Tris, pH 8.5 Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.99 D171A the mutant of isoform Arb43B displays no measurable activity Bacillus subtilis
3.2.1.99 D38A the mutant of isoform Arb43B displays no measurable activity Bacillus subtilis
3.2.1.99 E224A the mutant of isoform Arb43B displays no measurable activity Bacillus subtilis
3.2.1.99 H318A the mutant of isoform Arb43B displays a drastic decrease in enzymatic activity Bacillus subtilis
3.2.1.99 H318Q enzymatic activity is completely lost in this mutant Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 EGTA isoform Arb43B shows a drastic decrease in the activity of the enzyme in the presence of 1 mm EGTA Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.99 0.076
-
linear 1,5-alpha-L-arabinan mutant H318A of isoform Arb43B, pH and temperature not specified in the publication Bacillus subtilis
3.2.1.99 0.111
-
linear 1,5-alpha-L-arabinan wild type isoform Arb43B, pH and temperature not specified in the publication Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.99 Ca2+ isoform Arb43B possesses a cluster containing a calcium ion in the catalytic domain Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Bacillus subtilis P42293
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99 His-Trap column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 linear 1,5-alpha-L-arabinan + H2O
-
Bacillus subtilis L-arabinose + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 ABN
-
Bacillus subtilis
3.2.1.99 Arb43A isoform, formally named AbnA Bacillus subtilis
3.2.1.99 Arb43B isoform, formally named Abn2 Bacillus subtilis
3.2.1.99 endo-1,5-alpha-L-arabinanase
-
Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.99 3.1
-
linear 1,5-alpha-L-arabinan mutant H318A of isoform Arb43B, pH and temperature not specified in the publication Bacillus subtilis
3.2.1.99 191.6
-
linear 1,5-alpha-L-arabinan wild type isoform Arb43B, pH and temperature not specified in the publication Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.99 0.00004
-
linear 1,5-alpha-L-arabinan mutant H318A of isoform Arb43B, pH and temperature not specified in the publication Bacillus subtilis
3.2.1.99 0.00172
-
linear 1,5-alpha-L-arabinan wild type isoform Arb43B, pH and temperature not specified in the publication Bacillus subtilis