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Literature summary extracted from

  • Nakai, H.; Baumann, M.J.; Petersen, B.O.; Westphal, Y.; Hachem, M.A.; Dilokpimol, A.; Duus, J.?.; Schols, H.A.; Svensson, B.
    Aspergillus nidulans alpha-galactosidase of glycoside hydrolase family 36 catalyses the formation of alpha-galacto-oligosaccharides by transglycosylation (2010), FEBS J., 277, 3538-3551.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expressed in Escherichia coli as a His-tagged fusion protein Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.27
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5, 37°C Aspergillus nidulans
3.2.1.22 12
-
 melibiose pH 5, 37°C Aspergillus nidulans
3.2.1.22 15
-
 raffinose pH 5, 37°C Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 83000
-
 SDS-PAGE Aspergillus nidulans
3.2.1.22 335000
-
 gel filtration Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Aspergillus nidulans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Aspergillus nidulans 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 melibiose + H2O
-
 Aspergillus nidulans D-galactose + D-glucose
-
 ?
3.2.1.22 raffinose + H2O
-
 Aspergillus nidulans sucrose + alpha-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 tetramer 4 * 83000, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 AglC
-
 Aspergillus nidulans
3.2.1.22 alpha-galactosidase
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 50
-
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 45
-
 retains more than 95% activity after 15 min incubation up to 45°C at pH 5.0 Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 586
-
 raffinose pH 5, 37°C Aspergillus nidulans
3.2.1.22 1067
-
 melibiose pH 5, 37°C Aspergillus nidulans
3.2.1.22 1278
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5, 37°C Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5
-
-
 Aspergillus nidulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 3.6 9.9
-
 Aspergillus nidulans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.22 39.1
-
 raffinose pH 5, 37°C Aspergillus nidulans
3.2.1.22 88.9
-
 melibiose pH 5, 37°C Aspergillus nidulans
3.2.1.22 4730
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5, 37°C Aspergillus nidulans