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Literature summary extracted from

  • Cook, N.; Cammack, R.
    Purification and characterization of the rotenone-insensitive NADH dehydrogenase of mitochondria from Arum maculatum (1984), Eur. J. Biochem., 141, 573-577.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.9 flavone
-
 Arum maculatum
1.6.5.9 additional information the enzyme is not inhibited by rotenone, and activity is not affected by EDTA (1 mM), EGTA (1 mM) or Ca2+ or Mg2+ (1 mM) Arum maculatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.9 0.028
-
 NADH at 25°C and pH 7.2 Arum maculatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.9 mitochondrion
-
 Arum maculatum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.9 33900
-
 the rotenone-insensitive NADH-ubiquinone-1 reductase activity eluted by 0.5 M NaCl displays three major bands on SDS-PAGE of 55000, 39200 and 33900 Da Arum maculatum
1.6.5.9 39200
-
 the rotenone-insensitive NADH-ubiquinone-1 reductase activity eluted by 0.5 M NaCl displays three major bands on SDS-PAGE of 55000, 39200 and 33900 Da Arum maculatum
1.6.5.9 55000
-
 the rotenone-insensitive NADH-ubiquinone-1 reductase activity eluted by 0.5 M NaCl displays three major bands on SDS-PAGE of 55000, 39200 and 33900 Da Arum maculatum

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.9 Arum maculatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.9 5'-ADP-Sepharose 4B column chromatography Arum maculatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.9 3.7
-
 crude enzyme, using ubiquinone-1 as substrate, at 25°C and pH 7.2 Arum maculatum
1.6.5.9 69.2
-
 after 18.7fold purification, using ubiquinone-1 as substrate, at 25°C and pH 7.2 Arum maculatum
1.6.5.9 3372
-
 crude enzyme, using ubiquinone-1 as substrate, at 25°C and pH 7.2 Arum maculatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.9 2 ferricyanide + NADH 15% activity compared to ubiquinone-1 Arum maculatum 2 ferrocyanide + NAD+ + H+
-
 ?
1.6.5.9 additional information no activity with cytochrome c Arum maculatum ?
-
 ?
1.6.5.9 NADH + H+ + 2,6-dichloroindophenol 17% activity compared to ubiquinone-1 Arum maculatum NAD+ + reduced 2,6-dichloroindophenol
-
 ?
1.6.5.9 NADH + H+ + duroquinone 49% activity compared to ubiquinone-1 Arum maculatum NAD+ + duroquinol
-
 ?
1.6.5.9 NADH + H+ + juglone 29% activity compared to ubiquinone-1 Arum maculatum NAD+ + ?
-
 ?
1.6.5.9 NADH + H+ + menadione 47% activity compared to ubiquinone-1 Arum maculatum NAD+ + menadiol
-
 ?
1.6.5.9 NADH + H+ + ubiquinone-1 100% activity Arum maculatum NAD+ + ubiquinol-1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.9 rotenone-insensitive NADH dehydrogenase
-
 Arum maculatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.9 7.2
-
 pH-optimum around pH 7.2 Arum maculatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.9 FAD
-
 Arum maculatum
1.6.5.9 additional information no iron-sulfur centers Arum maculatum
1.6.5.9 NADH the partially purified enzyme is specific for NADH above pH 7.0 Arum maculatum
1.6.5.9 NADPH below pH 7.0 NADPH is slowly oxidized Arum maculatum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.6.5.9 0.175
-
 at 25°C and pH 7.2 Arum maculatum flavone