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Literature summary extracted from

  • Chhokar, V.; Seem, S.; Beniwal, V.; Salar, R.; Nehra, K.; Kumar, A.; Rana, J.
    Purification and characterization of extracellular tannin acyl hydrolase from Aspergillus heteromorphus MTCC 8818 (2010), Biotechnol. Bioprocess Eng., 15, 793-799.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 sodium lauryl sulfate 45% increase of activity at 1% (v/v) Aspergillus heteromorphus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 1,10-phenanthroline 42% inhibition at 1 mM Aspergillus heteromorphus
3.1.1.20 4-Aminobenzoic acid 81.9% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Ag+ 57% residual activity at 5 mM Aspergillus heteromorphus
3.1.1.20 Benzene 98% residual activity at 5% (v/v) Aspergillus heteromorphus
3.1.1.20 benzoic acid 83.1% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 beta-mercaptoethanol 59.4% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Cd2+ 57% residual activity at 5 mM Aspergillus heteromorphus
3.1.1.20 Co2+ 87.6% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 EDTA 35% inhibition at 1 mM Aspergillus heteromorphus
3.1.1.20 ethanol 80.2% residual activity at 5% (v/v) Aspergillus heteromorphus
3.1.1.20 gallic acid 82.1% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Hg2+ 56% residual activity at 5 mM Aspergillus heteromorphus
3.1.1.20 Isopropanol 96.3% residual activity at 5% (v/v) Aspergillus heteromorphus
3.1.1.20 K+ 73.3% residual activity at 5 mM Aspergillus heteromorphus
3.1.1.20 methanol 95.8% residual activity at 5% (v/v) Aspergillus heteromorphus
3.1.1.20 Mg2+ 77.5% residual activity at 5 mM Aspergillus heteromorphus
3.1.1.20 Mn2+ 79% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 additional information not inhibited by iodoacetamide Aspergillus heteromorphus
3.1.1.20 N-bromosuccinimide 73.9% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Na+ 89.1% residual activity at 5 mM Aspergillus heteromorphus
3.1.1.20 phenylmethylsulfonyl fluoride 86.1% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 propyl gallate 85.1% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 pyrogallol 95% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Sodium azide 82.8% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Sodium bisulfite 81.3% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Sodium thioglycolate 83.1% residual activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Triton X-100 21.9% inhibition at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 Tween 20 30.8% inhibition at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 Tween 80 27.4% inhibition at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 Zn2+ 80% residual activity at 5 mM Aspergillus heteromorphus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.20 extracellular
-
 Aspergillus heteromorphus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ 105.6% activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Cu+ 108.6% activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Cu2+ 106.2% activity at 1 mM Aspergillus heteromorphus
3.1.1.20 Fe2+ 107.6% activity at 1 mM Aspergillus heteromorphus
3.1.1.20 additional information Ba2+ has no significant effect on tannase activity up to 3 mM Aspergillus heteromorphus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.20 benzene 116.7% activity at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 Butanol 115.5% residual activity at 5% (v/v) Aspergillus heteromorphus
3.1.1.20 DMSO 122.4% residual activity at 5% (v/v) Aspergillus heteromorphus
3.1.1.20 Ethanol 104.4% residual activity at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 Glycerol 118.2% residual activity at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 isopropanol 106.6% activity at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 Methanol 103.2% residual activity at 1% (v/v) Aspergillus heteromorphus
3.1.1.20 toluene 119.4% residual activity at 1% (v/v) Aspergillus heteromorphus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus heteromorphus
-
-
-
3.1.1.20 Aspergillus heteromorphus MTCC 8818
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 ammonium sulfate precipitation and DEAE-cellulose column chromatography (overall purification of 39.74fold with a yield of 19.29%) Aspergillus heteromorphus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 0.575
-
 crude extract, pH 5.5, 50°C Aspergillus heteromorphus
3.1.1.20 22.85
-
 after 39.74fold purification, pH 5.5, 50°C Aspergillus heteromorphus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Aspergillus heteromorphus gallic acid + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus heteromorphus MTCC 8818 gallic acid + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus heteromorphus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 50
-
-
 Aspergillus heteromorphus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5.5
-
-
 Aspergillus heteromorphus