EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.2.8 | expression of maltose-binding protein fusion HepI, MBP-HepI, in Escherichia coli | Pedobacter heparinus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.2.8 | C297S | site-directed mutagenesis, the mutant suppresses the dimerization and shows 70% reduced activity compared to the wild-type enzyme | Pedobacter heparinus |
EC Number | General Stability | Organism |
---|---|---|
4.2.2.8 | after freeze-thawing, 97% of maximal activity remains | Pedobacter heparinus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.8 | alpha-lactose | - |
Pedobacter heparinus | |
4.2.2.8 | Ca2+ | - |
Pedobacter heparinus | |
4.2.2.8 | Dextran | - |
Pedobacter heparinus | |
4.2.2.8 | DTT | suppresses the dimerization | Pedobacter heparinus | |
4.2.2.8 | glycerol | - |
Pedobacter heparinus | |
4.2.2.8 | PEG 20000 | - |
Pedobacter heparinus | |
4.2.2.8 | PEG 8000 | - |
Pedobacter heparinus | |
4.2.2.8 | sucrose | - |
Pedobacter heparinus | |
4.2.2.8 | trehalose | - |
Pedobacter heparinus | |
4.2.2.8 | Triton X-100 | - |
Pedobacter heparinus | |
4.2.2.8 | Tween 80 | - |
Pedobacter heparinus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.8 | additional information | - |
additional information | kinetic modeling based on inactivation data, overview | Pedobacter heparinus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.8 | Ca2+ | activates | Pedobacter heparinus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.8 | Pedobacter heparinus | - |
formerly Flavobacterium heparinum | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.8 | recombinant maltose-binding protein fusion HepI, MBP-HepI, from Escherichia coli by affinity and anion exchange chromatography, followed by gel filtration to apparent homogeneity | Pedobacter heparinus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
4.2.2.8 | temperature-induced reactivation of MBP-HepI, when the temperature is lowered from 35°C to 4°C, the rate constant of unfolding decreases by 6000times while that of refolding decreases by only 600times, MBP-HepI undergoes reactivation during the cooling treatment at 4°C after incubation at 35°C | Pedobacter heparinus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.2.8 | 149 | - |
purified recombinant HepI, pH 7.5, 30°C | Pedobacter heparinus |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.2.8 | 4°C, purified recombinant MBP-HEPI, 1 week, 95% remaining activity | Pedobacter heparinus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.8 | additional information | cleavage/degradation of heparin forming unsaturated uronic acid | Pedobacter heparinus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.8 | More | three-dimensional model structure, modelling based on the known crystal structure of Bacteroides thetaiotaomicron HepI, overview | Pedobacter heparinus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.8 | heparinase I | - |
Pedobacter heparinus |
4.2.2.8 | HepI | - |
Pedobacter heparinus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.8 | 30 | - |
- |
Pedobacter heparinus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.8 | additional information | - |
the inactivation shows strong concentration dependence. Acceleration of the inactivation with the increase of enzyme concentration implies multi-molecular interactions in the thermal inactivation. Strong reversibility of the unfolding of MBP-HepI, overview | Pedobacter heparinus |
4.2.2.8 | 30 | - |
purified recombinant enzyme, half-life is 160 min | Pedobacter heparinus |
4.2.2.8 | 70 | - |
purified recombinant enzyme, loss of 97% activity within 1 min | Pedobacter heparinus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.8 | 7.5 | - |
assay at | Pedobacter heparinus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.2.8 | additional information | intermolecular disulfide bond formation in HepI important for catalysis, overview | Pedobacter heparinus |