EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.153 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.153 | D81N | 24% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.5.1.153 | D81N/D82N | inactive | Mycobacterium tuberculosis |
2.5.1.153 | D81N/D85N | inactive | Mycobacterium tuberculosis |
2.5.1.153 | D82N | 21% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.5.1.153 | D82N/D85N | inactive | Mycobacterium tuberculosis |
2.5.1.153 | D85N | 37% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.153 | Cu2+ | 17% activity at 1 mM | Mycobacterium tuberculosis | |
2.5.1.153 | Fe2+ | 20% activity at 1 mM | Mycobacterium tuberculosis | |
2.5.1.153 | Mn2+ | 13% activity at 1 mM | Mycobacterium tuberculosis | |
2.5.1.153 | Ni2+ | 35% activity at 1 mM | Mycobacterium tuberculosis | |
2.5.1.153 | Zn2+ | 10% activity at 1 mM | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.153 | 0.0254 | - |
tuberculosinyl diphosphate | mutant enzyme D82N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.0271 | - |
tuberculosinyl diphosphate | wild type enzyme, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.0273 | - |
tuberculosinyl diphosphate | mutant enzyme D85N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.0396 | - |
tuberculosinyl diphosphate | mutant enzyme D81N, at pH 7.0, 45°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.153 | Mg2+ | essential for activity, maximum activity at 1 mM Mg2+ | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.153 | 30000 | - |
2 * 30000, SDS-PAGE | Mycobacterium tuberculosis |
2.5.1.153 | 60000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.153 | Mycobacterium tuberculosis | - |
- |
- |
2.5.1.153 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.153 | Ni-NTA column chromatography, amylase column chromatography, and G3000SWXL gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.153 | 2 tuberculosinyl diphosphate + 2 H2O | - |
Mycobacterium tuberculosis | (13R)-isotuberculosinol + (13S)-isotuberculosinol + 2 diphosphate | isotuberculosinol is also named nosyberkol, the product ratio of (13R)-isotuberculosinol and (13S)-isotuberculosinol is 1:3 | ? | |
2.5.1.153 | 2 tuberculosinyl diphosphate + 2 H2O | - |
Mycobacterium tuberculosis H37Rv | (13R)-isotuberculosinol + (13S)-isotuberculosinol + 2 diphosphate | isotuberculosinol is also named nosyberkol, the product ratio of (13R)-isotuberculosinol and (13S)-isotuberculosinol is 1:3 | ? | |
2.5.1.153 | additional information | no activity with geranyl diphosphate, farnesyl diphosphate or geranylgeranyl diphosphate | Mycobacterium tuberculosis | ? | - |
? | |
2.5.1.153 | additional information | no activity with geranyl diphosphate, farnesyl diphosphate or geranylgeranyl diphosphate | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis | tuberculosinol + diphosphate | - |
? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | tuberculosinol + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.153 | homodimer | 2 * 30000, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.153 | Rv3378c protein | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.153 | 45 | - |
- |
Mycobacterium tuberculosis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.153 | 35 | 45 | no activity at temperatures higher than 50°C | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.153 | 0.0000998 | - |
tuberculosinyl diphosphate | mutant enzyme D82N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.00018 | - |
tuberculosinyl diphosphate | mutant enzyme D81N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.000192 | - |
tuberculosinyl diphosphate | mutant enzyme D85N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.000517 | - |
tuberculosinyl diphosphate | wild type enzyme, at pH 7.0, 45°C | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.153 | 7 | - |
- |
Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.153 | 5.5 | 9 | - |
Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.153 | 0.00398 | - |
tuberculosinyl diphosphate | mutant enzyme D82N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.0045 | - |
tuberculosinyl diphosphate | mutant enzyme D81N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.007 | - |
tuberculosinyl diphosphate | mutant enzyme D85N, at pH 7.0, 45°C | Mycobacterium tuberculosis | |
2.5.1.153 | 0.0191 | - |
tuberculosinyl diphosphate | wild type enzyme, at pH 7.0, 45°C | Mycobacterium tuberculosis |