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Literature summary extracted from

  • Fukuta, Y.; Nanda, S.; Kato, Y.; Yurimoto, H.; Sakai, Y.; Komeda, H.; Asano, Y.
    Characterization of a new (R)-hydroxynitrile lyase from the Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris (2011), Biosci. Biotechnol. Biochem., 75, 214-220.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10 expresion in Pichia pastoris Prunus mume
4.1.2.10 expressed in Pichia pastoris strain GS115 Prunus mume

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.10 2-Iodoacetamide 1 mM, 9% residual activity; 9.0% residual activity at 1 mM Prunus mume
4.1.2.10 2-mercaptoethanol 87.3% residual activity at 1 mM Prunus mume
4.1.2.10 Ag+ 1 mM, 8% residual activity; 8.2 residual activity at 1 mM Prunus mume
4.1.2.10 diethyldicarbonate 35.4% residual activity at 1 mM Prunus mume
4.1.2.10 Hg2+ 1 mM, 9% residual activity; 9.0% residual activity at 1 mM Prunus mume
4.1.2.10 iodoacetic acid 1 mM, 8% residual activity; 8.0% residual activity at 1 mM Prunus mume
4.1.2.10 additional information not inhibited by EDTA, EGTA, sodium fluoride, o-phenanthroline, 2,4-dinitro-1-thiocyanate, NaN3, dithiothreitol, phenylhydrazine, Triton X-100, 2,2'-bipyridyl, N-ethylmaleimide, and D-cycloserine Prunus mume
4.1.2.10 NH2OH 82.7% residual activity at 1 mM Prunus mume
4.1.2.10 p-chloromercuribenzoic acid 58.6% residual activity at 10 mM Prunus mume
4.1.2.10 phenylmethylsulfonyl fluoride 68.5% residual activity at 1 mM Prunus mume

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 5.3
-
 benzaldehyde pH 5.0, 25°C Prunus mume
4.1.2.10 5.3
-
 benzaldehyde isoform HNL2, at pH 4.5 and 25°C Prunus mume

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 57000
-
 gel filtration Prunus mume
4.1.2.10 58100
-
 1 * 58100, recombinant (R)-HNL, SDS-PAGE Prunus mume
4.1.2.10 58100
-
 1 * 58100, SDS-PAGE Prunus mume
4.1.2.10 62000
-
 1 * 62000, non-glycosylated isoform HNL2, SDS-PAGE Prunus mume
4.1.2.10 62000
-
 x * 62751, calculated, x * 62000, SDS-PAGE of deglycosylated protein Prunus mume
4.1.2.10 62751
-
 1 * 62751, isoform HNL2, calculated from amino acid sequence Prunus mume
4.1.2.10 62751
-
 x * 62751, calculated, x * 62000, SDS-PAGE of deglycosylated protein Prunus mume
4.1.2.10 97000
-
 1 * 97000, glycosylated isoform HNL2, SDS-PAGE Prunus mume

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus mume
-
-
-
4.1.2.10 Prunus mume B9X0I1
-
-
4.1.2.10 Prunus mume B9X0I2 cultivar Nanko
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.2.10 flavoprotein enzyme shows a typical flavoprotein spectrum with absorption maxima at 389 nm nd 463 nm Prunus mume
4.1.2.10 glycoprotein molecular mass 97000 Da, mass of deglycosylated protein 62000 Da Prunus mume

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Prunus mume
4.1.2.10 ammonium sulfate precipitation, DEAE Toyopearl column chromatography, phenyl Toyopearl column chromatography, and Superdex 200 gel filtration Prunus mume

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 seedling
-
 Prunus mume
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.10 2 20 pH 5.0, 25°C Prunus mume
4.1.2.10 9
-
 (R)-HNL from crude extract, in 0.3 mM citrate buffer, at pH 5.0 and 25°C Prunus mume
4.1.2.10 129
-
 isoform HNL2 after purification, in 0.3 mM citrate buffer, at pH 5.0 and 25°C Prunus mume
4.1.2.10 129
-
 pH 5.0, 25°C Prunus mume
4.1.2.10 220
-
 (R)-HNL after 24.4fold purification, in 0.3 mM citrate buffer, at pH 5.0 and 25°C Prunus mume

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 cyanide + benzaldehyde
-
 Prunus mume (R)-mandelonitrile
-
 ?
4.1.2.10 cyanide + benzaldehyde
-
 Prunus mume (R)-mandelonitrile
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.10 ? x * 62751, calculated, x * 62000, SDS-PAGE of deglycosylated protein Prunus mume
4.1.2.10 monomer 1 * 58100, recombinant (R)-HNL, SDS-PAGE Prunus mume
4.1.2.10 monomer 1 * 62000, non-glycosylated isoform HNL2, SDS-PAGE Prunus mume
4.1.2.10 monomer 1 * 62751, isoform HNL2, calculated from amino acid sequence Prunus mume
4.1.2.10 monomer 1 * 97000, glycosylated isoform HNL2, SDS-PAGE Prunus mume
4.1.2.10 monomer 1 * 58100, SDS-PAGE Prunus mume

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-HNL
-
 Prunus mume
4.1.2.10 (R)-hydroxynitrile lyase
-
 Prunus mume
4.1.2.10 HNL2
-
 Prunus mume
4.1.2.10 HNL2 isoform Prunus mume

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 25 35
-
 Prunus mume

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 4.5
-
-
 Prunus mume

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.10 FAD
-
 Prunus mume