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Literature summary extracted from

  • Chau, T.T.; Ishigaki, M.; Kataoka, T.; Taketani, S.
    Porcine ferrochelatase: the relationship between iron-removal reaction and the conversion of heme to Zn-protoporphyrin (2010), Biosci. Biotechnol. Biochem., 74, 1415-1420.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.98.1.1 expressed in Escherichia coli BL21 cells Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.98.1.1 0.0011
-
 Zn2+ pH 8.0, 37°C Sus scrofa
4.98.1.1 0.0057
-
 hemin pH 5.5, 45°C Sus scrofa
4.98.1.1 0.0066
-
 mesoporphyrin IX pH 8.0, 37°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.98.1.1 mitochondrion inner mitochondrial membrane Sus scrofa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.98.1.1 42000
-
 x * 42000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.98.1.1 protoheme + H+ Sus scrofa
-
 protoporphyrin + Fe2+
-
 r
4.98.1.1 protoporphyrin + Fe2+ Sus scrofa the kcat of the forward reaction is about 11fold higher than the reverse reaction protoheme + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.98.1.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.98.1.1 Ni2+-NTA bead column chromatography, gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.98.1.1 liver highest activity Sus scrofa
-
4.98.1.1 LLC-PK1 cell
-
 Sus scrofa
-
4.98.1.1 muscle
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.98.1.1 heme + Zn2+
-
 Sus scrofa Zn-protoporphyrin + H+
-
 ?
4.98.1.1 hemin + H+
-
 Sus scrofa mesoporphyrin IX + Zn2+
-
 r
4.98.1.1 mesoporphyrin IX + Zn2+
-
 Sus scrofa Zn2+-mesoporphyrin + 2 H+
-
 r
4.98.1.1 protoheme + H+
-
 Sus scrofa protoporphyrin + Fe2+
-
 r
4.98.1.1 protoporphyrin + Fe2+ the kcat of the forward reaction is about 11fold higher than the reverse reaction Sus scrofa protoheme + H+
-
 r

Subunits

EC Number Subunits Comment Organism
4.98.1.1 ? x * 42000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
4.98.1.1 FeCH
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.98.1.1 0.52
-
 hemin pH 5.5, 45°C Sus scrofa
4.98.1.1 6.7
-
 Zn2+ pH 8.0, 37°C Sus scrofa
4.98.1.1 6.7
-
 mesoporphyrin IX pH 8.0, 37°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.98.1.1 5.5 6 pH optimum for zinc removal Sus scrofa
4.98.1.1 7.5 8 pH optimum for zinc insertion Sus scrofa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.98.1.1 0.000092
-
 hemin pH 5.5, 45°C Sus scrofa
4.98.1.1 0.00101
-
 mesoporphyrin IX pH 8.0, 37°C Sus scrofa
4.98.1.1 0.00525
-
 Zn2+ pH 8.0, 37°C Sus scrofa