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Literature summary extracted from
- Understanding the mode of action of ThDP in benzoylformate decarboxylase (2010), Biopolymers, 93, 32-46.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.1.7 | H281A | inactive | Pseudomonas putida |
| 4.1.1.7 | H281F | the mutation leads to a 5fold decrease in the enzyme activity | Pseudomonas putida |
| 4.1.1.7 | H70A | inactive | Pseudomonas putida |
| 4.1.1.7 | H70L | the mutant shows 30% decrease in the enzyme activity | Pseudomonas putida |
| 4.1.1.7 | H70T | the mutant shows 30% decrease in the enzyme activity | Pseudomonas putida |
| 4.1.1.7 | L461A | the mutant has decreased activity compared to the wild type enzyme, the enantioselectivity of the (S)-2-hydroxypropiophenone formation is increased by 6%. In the case of propionaldehyde, the enzyme activity increases considerably from 6% with wild type BFD to 21.5%. With butyraldehyde the already low enzyme activity does not change | Pseudomonas putida |
| 4.1.1.7 | L461G | the mutant has decreased activity compared to the wild type enzyme, the enantioselectivity of the (S)-2-hydroxypropiophenone formation is increased by 6%. In the case of propionaldehyde, the enzyme activity increases considerably from 6% with wild type BFD to 23%. With butyraldehyde the already low enzyme activity does not change | Pseudomonas putida |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.7 | Mg2+ | contains Mg2+ | Pseudomonas putida |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.7 | Pseudomonas putida | P20906 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.7 | benzaldehyde + acetaldehyde | - |
Pseudomonas putida | (S)-2-hydroxypropiophenone | i.e. acyloin | ? | |
| 4.1.1.7 | benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
| 4.1.1.7 | butyraldehyde | - |
Pseudomonas putida | ? | - |
? | |
| 4.1.1.7 | propionaldehyde | - |
Pseudomonas putida | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.7 | BFD | - |
Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.7 | thiamine diphosphate | - |
Pseudomonas putida | |
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Release 2023.1 (January 2023)
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