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Literature summary extracted from

  • Perumal Samy, R.; Gopalakrishnakone, P.; Bow, H.; Puspharaj, P.N.; Chow, V.T.
    Identification and characterization of a phospholipase A2 from the venom of the Saw-scaled viper: Novel bactericidal and membrane damaging activities (2010), Biochimie, 92, 1854-1866.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.4 medicine the purified enzyme shows strong bactericidal activity against Burkholderia pseudomallei (minimum inhibitory concentration above 0.015 mg/ml) and Enterobacter aerogenes (minimum inhibitory concentration above 0.03 mg/ml) Echis carinatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 13862
-
 x * 13862, MALDI-TOF mass spectrometry Echis carinatus
3.1.1.4 15000
-
 x * 15000, SDS-PAGE Echis carinatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Echis carinatus
-
 saw-scaled viper
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 C8 Sepharose column chromatography, C18 Sepharose column chromatography, and Superdex G-75 gel filtration Echis carinatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 venom
-
 Echis carinatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 1034
-
 crude venom, pH and temperature not specified in the publication Echis carinatus
3.1.1.4 2312
-
 purified enzyme, pH and temperature not specified in the publication Echis carinatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 phosphatidylcholine + H2O
-
 Echis carinatus 1-acylglycerophosphocholine + fatty acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 ? x * 15000, SDS-PAGE Echis carinatus
3.1.1.4 ? x * 13862, MALDI-TOF mass spectrometry Echis carinatus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 PLA2
-
 Echis carinatus
3.1.1.4 toxin I
-
 Echis carinatus
3.1.1.4 Tx-1
-
 Echis carinatus

General Information

EC Number General Information Comment Organism
3.1.1.4 physiological function the phospholipase A2 toxin I displays a heparin binding C-terminal region, which is probably responsible for the cytotoxic and bactericidal effects. Toxin I is able to induce cytotoxicity on THP-1 cells in vitro as well as lethality in BALB/c mice. Toxin II also induces mild myotoxic effects on mouse skin, but is devoid of hemolytic effects on human erythrocytes up to 0.5 mg/ml Echis carinatus