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Literature summary extracted from

  • Patin, D.; Boniface, A.; Kovac, A.; Hervé, M.; Dementin, S.; Barreteau, H.; Mengin-Lecreulx, D.; Blanot, D.
    Purification and biochemical characterization of Mur ligases from Staphylococcus aureus (2010), Biochimie, 92, 1793-1800.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.7 expression in Escherichia coli Staphylococcus aureus
6.3.2.8 expression in Escherichia coli Staphylococcus aureus
6.3.2.9 expression in Escherichia coli Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.7 0.087
-
 UDP-N-acetylmuramoyl-L-Ala-D-Glu pH 8.6, 37°C Staphylococcus aureus
6.3.2.7 0.53
-
 ATP pH 8.6, 37°C Staphylococcus aureus
6.3.2.7 0.55
-
 L-Lys pH 8.6, 37°C Staphylococcus aureus
6.3.2.8 0.28
-
 UDP-N-acetylmuramate pH 8.6, 37°C Staphylococcus aureus
6.3.2.8 0.44
-
 L-Ala pH 8.6, 37°C Staphylococcus aureus
6.3.2.8 2
-
 ATP pH 8.6, 37°C Staphylococcus aureus
6.3.2.9 0.041
-
 UDP-N-acetylmuramoyl-L-Ala pH 8.8, 37°C Staphylococcus aureus
6.3.2.9 0.13
-
 D-Glu pH 8.8, 37°C Staphylococcus aureus
6.3.2.9 5.4
-
 ATP pH 8.8, 37°C Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.7 Mg2+ required, optimal concentration 15 mM Staphylococcus aureus
6.3.2.8 Mg2+ required, optimal concentration 5 mM Staphylococcus aureus
6.3.2.9 Mg2+ required, optimal concentration 15 mM Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.7 Staphylococcus aureus
-
-
-
6.3.2.8 Staphylococcus aureus
-
-
-
6.3.2.9 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.7 recombinant protein Staphylococcus aureus
6.3.2.8 recombinant protein Staphylococcus aureus
6.3.2.9 recombinant protein Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.7 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys
-
 Staphylococcus aureus ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys
-
 ?
6.3.2.7 additional information enzyme is specific for L-lysine and does not accept meso-diaminopimelic acid as substrate. The inverse and strict substrate specificities of the two MurE orthologues are responsible for the presence of exclusively mesodiaminopimelic acid and L-lysine at the third position of the peptide in the peptidoglycans of Escherichia coli and Staphylococcus aureus, respectively Staphylococcus aureus ?
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + 2-amino-N-butyric acid
-
 Staphylococcus aureus ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-butyric acid
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala accepts L-Ala, L-Ser and Gly as substrates, with a strong preference for L-Ala Staphylococcus aureus ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
6.3.2.9 ATP + UDP-N-acetylmuramoyl-L-Ala + D-Glu
-
 Staphylococcus aureus ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.7 8.4 9.2
-
 Staphylococcus aureus
6.3.2.8 8.2 8.8
-
 Staphylococcus aureus
6.3.2.9 8.4 9.6
-
 Staphylococcus aureus