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Literature summary extracted from

  • Beassoni, P.R.; Otero, L.H.; Boetsch, C.; Domenech, C.E.; Gonzalez-Nilo, F.D.; Lisa, A.T.
    Site-directed mutations and kinetic studies show key residues involved in alkylammonium interactions and reveal two sites for phosphorylcholine in Pseudomonas aeruginosa phosphorylcholine phosphatase (2011), Biochim. Biophys. Acta, 1814, 858-863.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.75 E42A site-directed mutagenesis Pseudomonas aeruginosa
3.1.3.75 E43A site-directed mutagenesis Pseudomonas aeruginosa
3.1.3.75 Y82A/Y83A/Y84A site-directed mutagenesis Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.75 phosphocholine substrate inhibition at high concentration Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.75 0.51
-
 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant mutant Y82A/Y83A/Y84A Pseudomonas aeruginosa
3.1.3.75 0.56
-
 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant mutant E43A Pseudomonas aeruginosa
3.1.3.75 1.5 2 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant wild-type enzyme Pseudomonas aeruginosa
3.1.3.75 1.9
-
 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant mutant E42A Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Mg2+ dependent on Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.75 phosphocholine + H2O Pseudomonas aeruginosa
-
 choline + phosphate
-
 ?
3.1.3.75 phosphoethanolamine + H2O Pseudomonas aeruginosa
-
 ethanolamine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Pseudomonas aeruginosa Q9HTR2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.75 10.1
-
 purified recombinant mutant Y82A/Y83A/Y84A, pH 5.0, 37°C, substrate 4-nitrophenyl phosphate Pseudomonas aeruginosa
3.1.3.75 59
-
 purified recombinant mutant E42A, pH 5.0, 37°C, substrate 4-nitrophenyl phosphate Pseudomonas aeruginosa
3.1.3.75 121
-
 purified recombinant mutant E43A, pH 5.0, 37°C, substrate 4-nitrophenyl phosphate Pseudomonas aeruginosa
3.1.3.75 132.7
-
 purified recombinant wild-type enzyme, pH 5.0, 37°C, substrate 4-nitrophenyl phosphate Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 4-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
 ?
3.1.3.75 phosphocholine + H2O
-
 Pseudomonas aeruginosa choline + phosphate
-
 ?
3.1.3.75 phosphoethanolamine + H2O
-
 Pseudomonas aeruginosa ethanolamine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.75 More the enzyme contains three HAD motifs, sequence comparisons, overview Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 More the enzyme belongs to the haloacid dehalogenase superfamily Pseudomonas aeruginosa
3.1.3.75 PChP
-
 Pseudomonas aeruginosa
3.1.3.75 phosphoethanolamine/phosphocholine phosphatase
-
 Pseudomonas aeruginosa
3.1.3.75 phosphorylcholine phosphatase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.75 37
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.75 6.7
-
 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant mutant Y82A/Y83A/Y84A Pseudomonas aeruginosa
3.1.3.75 38.8
-
 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant mutant E42A Pseudomonas aeruginosa
3.1.3.75 79.6
-
 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant mutant E43A Pseudomonas aeruginosa
3.1.3.75 87.1
-
 4-nitrophenyl phosphate pH 5.0, 37°C, recombinant wild-type enzyme Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.75 5
-
 assay at Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
3.1.3.75 additional information combination of molecular modeling, directed mutagenesis and enzyme kinetics to study the mode of interaction of PchP phosphatase with the choline moiety of the substrate Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.75 13.3
-
 4-nitrophenyl phosphate purified recombinant mutant Y82A/Y83A/Y84A, pH 5.0, 37°C Pseudomonas aeruginosa
3.1.3.75 21.4
-
 4-nitrophenyl phosphate purified recombinant mutant E42A, pH 5.0, 37°C Pseudomonas aeruginosa
3.1.3.75 57.2
-
 4-nitrophenyl phosphate purified recombinant wild-type enzyme, pH 5.0, 37°C Pseudomonas aeruginosa
3.1.3.75 141
-
 4-nitrophenyl phosphate purified recombinant mutant E43A, pH 5.0, 37°C Pseudomonas aeruginosa