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Literature summary extracted from

  • Erben, E.D.; Valguarnera, E.; Nardelli, S.; Chung, J.; Daum, S.; Potenza, M.; Schenkman, S.; Tellez-Inon, M.T.
    Identification of an atypical peptidyl-prolyl cis/trans isomerase from trypanosomatids (2010), Biochim. Biophys. Acta, 1803, 1028-1037.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.2.1.8 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.2.1.8 nucleus Par45 is mainly concentrated in the nucleus Trypanosoma cruzi 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.2.1.8 45400
-
 x * 45400, calculated from amino acid sequence Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.8 Trypanosoma cruzi B8XP93
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.2.1.8 Ni-NTA agarose column chromatography and Superdex 75 gel filtration Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.2.1.8 amastigote
-
 Trypanosoma cruzi
-
5.2.1.8 epimastigote
-
 Trypanosoma cruzi
-
5.2.1.8 trypomastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.8 additional information Par45 does not accelerate the cis/trans interconversion of acidic substrates containing Glu-Pro bonds Trypanosoma cruzi ?
-
 ?
5.2.1.8 N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide 6.9% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide
-
 ?
5.2.1.8 N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide 100% activity, Par45 shows a strong preference for a substrate with the basic Arg residue preceding Pro Trypanosoma cruzi N-succinyl-Ala-Arg-(trans)-Pro-Phe-4-nitroanilide
-
 ?
5.2.1.8 N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide 7.6% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide
-
 ?
5.2.1.8 N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide 26.7% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide
-
 ?
5.2.1.8 N-succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide 4.3% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Phe-(trans)-Pro-Phe-4-nitroanilide
-
 ?

Subunits

EC Number Subunits Comment Organism
5.2.1.8 ? x * 45400, calculated from amino acid sequence Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
5.2.1.8 Par45
-
 Trypanosoma cruzi
5.2.1.8 peptidyl-prolyl cis/trans isomerase
-
 Trypanosoma cruzi
5.2.1.8 PPIase
-
 Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
5.2.1.8 physiological function Par45 is a phosphorylation-independent parvulin required for normal cell proliferation in a unicellular eukaryotic cell Trypanosoma cruzi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.2.1.8 4.2
-
 N-succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
5.2.1.8 6.7
-
 N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
5.2.1.8 7.4
-
 N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
5.2.1.8 25.9
-
 N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
5.2.1.8 97.1
-
 N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi