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Literature summary extracted from

  • Bouvier, F.; Keller, Y.; DHarlingue, A.; Camara, B.
    Xanthophyll biosynthesis: Molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.) (1998), Biochim. Biophys. Acta, 1391, 320-328.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.24 expressed in Escherichia coli DH5alpha cells Capsicum annuum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.24 H171A inactive Capsicum annuum
1.14.15.24 H176A inactive Capsicum annuum
1.14.15.24 H184A inactive Capsicum annuum
1.14.15.24 H187A inactive Capsicum annuum
1.14.15.24 H188A inactive Capsicum annuum
1.14.15.24 H245A inactive Capsicum annuum
1.14.15.24 H250A inactive Capsicum annuum
1.14.15.24 H274A inactive Capsicum annuum
1.14.15.24 H275A inactive Capsicum annuum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.24 8-hydroxyquinoline 5% residual activity at 1 mM Capsicum annuum
1.14.15.24 cytochrome c addition of cytochrome c to the incubation medium inhibits hydroxylase activity Capsicum annuum
1.14.15.24 o-phenanthroline 10% residual activity at 1 mM Capsicum annuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.24 24000 26000 SDS-PAGE Capsicum annuum

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.24 Capsicum annuum O49814
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.24 Q-Sepharose column chromatography, Talon nickel-affinity resin column chromatography, and Mono Q column chromatography Capsicum annuum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.24 fruit
-
 Capsicum annuum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase Capsicum annuum beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase Capsicum annuum zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.24 beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase Capsicum annuum zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.24 carotenoid hydroxylase 2
-
 Capsicum annuum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.24 Ferredoxin the hydroxylase is strictly dependent upon reduced ferredoxin which cannot be replaced by NADPH Capsicum annuum