Literature summary extracted from
Bouvier, F.; Keller, Y.; DHarlingue, A.; Camara, B.
Xanthophyll biosynthesis: Molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.) (1998), Biochim. Biophys. Acta, 1391, 320-328.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.15.24 |
expressed in Escherichia coli DH5alpha cells |
Capsicum annuum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.15.24 |
H171A |
inactive |
Capsicum annuum |
1.14.15.24 |
H176A |
inactive |
Capsicum annuum |
1.14.15.24 |
H184A |
inactive |
Capsicum annuum |
1.14.15.24 |
H187A |
inactive |
Capsicum annuum |
1.14.15.24 |
H188A |
inactive |
Capsicum annuum |
1.14.15.24 |
H245A |
inactive |
Capsicum annuum |
1.14.15.24 |
H250A |
inactive |
Capsicum annuum |
1.14.15.24 |
H274A |
inactive |
Capsicum annuum |
1.14.15.24 |
H275A |
inactive |
Capsicum annuum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.15.24 |
8-hydroxyquinoline |
5% residual activity at 1 mM |
Capsicum annuum |
|
1.14.15.24 |
cytochrome c |
addition of cytochrome c to the incubation medium inhibits hydroxylase activity |
Capsicum annuum |
|
1.14.15.24 |
o-phenanthroline |
10% residual activity at 1 mM |
Capsicum annuum |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.15.24 |
24000 |
26000 |
SDS-PAGE |
Capsicum annuum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.24 |
Capsicum annuum |
O49814 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.24 |
Q-Sepharose column chromatography, Talon nickel-affinity resin column chromatography, and Mono Q column chromatography |
Capsicum annuum |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.14.15.24 |
fruit |
- |
Capsicum annuum |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.24 |
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 |
the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase |
Capsicum annuum |
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
|
1.14.15.24 |
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 |
the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase |
Capsicum annuum |
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O |
- |
? |
|
1.14.15.24 |
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 |
the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase |
Capsicum annuum |
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.15.24 |
carotenoid hydroxylase 2 |
- |
Capsicum annuum |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.24 |
Ferredoxin |
the hydroxylase is strictly dependent upon reduced ferredoxin which cannot be replaced by NADPH |
Capsicum annuum |
|