Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miyoshi-Akiyama, T.; Hayashi, M.; Unemoto, T.
    Purification and properties of cytochrome bo-type ubiquinol oxidase from a marine bacterium Vibrio alginolyticus (1993), Biochim. Biophys. Acta, 1141, 283-287.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.3 diphosphatidyl glycerol activates the enzyme with the optimum concentration at 0.04 mg/ml Vibrio alginolyticus
7.1.1.3 liponox DCH the activity is saturated at 0.01-0.02% (v/v) Liponox DCH Vibrio alginolyticus
7.1.1.3 additional information the enzyme is not activated by sodium cholate, phosphatidyl glycerol, phosphatidyl ethanolamine and soybean phospholipids Vibrio alginolyticus
7.1.1.3 n-dodecyl octaethyleneglycol monoether effective activator Vibrio alginolyticus
7.1.1.3 N-lauroylsarcosine effective activator Vibrio alginolyticus
7.1.1.3 Zwittergent 3-14 effective activator Vibrio alginolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.3 2-n-heptyl-4-hydroxyquinoline N-oxide
-
 Vibrio alginolyticus
7.1.1.3 KCN
-
 Vibrio alginolyticus
7.1.1.3 ZnSO4
-
 Vibrio alginolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.3 membrane
-
 Vibrio alginolyticus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.3 Cu2+ the oxidase contains copper atoms Vibrio alginolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.3 13000
-
 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus
7.1.1.3 36000
-
 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus
7.1.1.3 79000
-
 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.3 Vibrio alginolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.3 DEAE-Sephacel gel filtration, DEAE-5PW gel filtration, and Sephacryl S-300 gel filtration Vibrio alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.3 duroquinol + O2 + H+/in
-
 Vibrio alginolyticus duroquinone + H2O + H+/out
-
 ?
7.1.1.3 additional information horse heart reduced cytochrome c is not oxidized by this enzyme Vibrio alginolyticus ?
-
 ?
7.1.1.3 N,N,N',N'-tetramethylphenylene diamine + O2
-
 Vibrio alginolyticus ?
-
 ?
7.1.1.3 ubiquinol-1 + O2 + H+[side 1]
-
 Vibrio alginolyticus ubiquinone-1 + H2O + H+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.3 heterodimer 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE Vibrio alginolyticus

Synonyms

EC Number Synonyms Comment Organism
7.1.1.3 cytochrome bo-type ubiquinol oxidase
-
 Vibrio alginolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.3 cytochrome the oxidase contains cytochrome b, cytochrome o Vibrio alginolyticus
7.1.1.3 heme the purified oxidase contains both protoheme and heme O Vibrio alginolyticus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.1.1.3 0.0032
-
 in 25 mM potassium phosphate (pH 7.5), at 30°C Vibrio alginolyticus 2-n-heptyl-4-hydroxyquinoline N-oxide
7.1.1.3 0.0034
-
 in 25 mM potassium phosphate (pH 7.5), at 30°C Vibrio alginolyticus ZnSO4
7.1.1.3 0.0064
-
 in 25 mM potassium phosphate (pH 7.5), at 30°C Vibrio alginolyticus KCN

General Information

EC Number General Information Comment Organism
7.1.1.3 physiological function the bo-type ubiquinol oxidase is functioning as a proton pump Vibrio alginolyticus