Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Poliakov, E.; Parikh, T.; Ayele, M.; Kuo, S.; Chander, P.; Gentleman, S.; Redmond, T.M.
    Aromatic lipophilic spin traps effectively inhibit RPE65 isomerohydrolase activity (2011), Biochemistry, 50, 6739-6741.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.64 2,2-dimethyl-4-phenyl-2H-imidazole-1-oxide strong inhibition of isomerohydrolase activity (32% at 0.2 mM) in vitro Bos taurus
3.1.1.64 2-(2-carboxyethyl)-2-methyl-4-phenyl-2H-imidazole-1-oxide moderate inhibitory effect at 0.2 mM Bos taurus
3.1.1.64 additional information not inhibited by N-tert-butyl-(2-sulfophenyl)nitrone, alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone, NXY059, tert-butylhydroxylamine, and 2-methyl-2-nitrosopropane Bos taurus
3.1.1.64 n-benzylidene-tert-butylamine 14% inhibition at 0.2 mM Bos taurus
3.1.1.64 N-tert-butyl-alpha-phenylnitrone strong uncompetitive inhibition (52%) of isomerohydrolase activity in vitro at 0.2 mM Bos taurus
3.1.1.64 nitrosobenzene strong inhibition of isomerohydrolase activity in vitro at 0.2 mM Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.64 microsome
-
 Bos taurus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.64 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.64 retinal pigment epithelial cell
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.64 all-trans-retinyl ester + H2O
-
 Bos taurus 11-cis-retinol + a fatty acid RPE65 does not specifically produce 11-cis-retinol only but also 13-cis-retinol ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.64 Rpe65
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.64 0.061
-
 N-tert-butyl-alpha-phenylnitrone pH and temperature not specified in the publication Bos taurus