BRENDA - Enzyme Database show

Aromatic lipophilic spin traps effectively inhibit RPE65 isomerohydrolase activity

Poliakov, E.; Parikh, T.; Ayele, M.; Kuo, S.; Chander, P.; Gentleman, S.; Redmond, T.M.; Biochemistry 50, 6739-6741 (2011)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
2,2-dimethyl-4-phenyl-2H-imidazole-1-oxide
strong inhibition of isomerohydrolase activity (32% at 0.2 mM) in vitro
Bos taurus
3.1.1.64
2-(2-carboxyethyl)-2-methyl-4-phenyl-2H-imidazole-1-oxide
moderate inhibitory effect at 0.2 mM
Bos taurus
3.1.1.64
additional information
not inhibited by N-tert-butyl-(2-sulfophenyl)nitrone, alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone, NXY059, tert-butylhydroxylamine, and 2-methyl-2-nitrosopropane
Bos taurus
3.1.1.64
n-benzylidene-tert-butylamine
14% inhibition at 0.2 mM
Bos taurus
3.1.1.64
N-tert-butyl-alpha-phenylnitrone
strong uncompetitive inhibition (52%) of isomerohydrolase activity in vitro at 0.2 mM
Bos taurus
3.1.1.64
nitrosobenzene
strong inhibition of isomerohydrolase activity in vitro at 0.2 mM
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
microsome
-
Bos taurus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelial cell
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
714267
Bos taurus
11-cis-retinol + a fatty acid
RPE65 does not specifically produce 11-cis-retinol only but also 13-cis-retinol
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.1.64
0.061
-
N-tert-butyl-alpha-phenylnitrone
pH and temperature not specified in the publication
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
2,2-dimethyl-4-phenyl-2H-imidazole-1-oxide
strong inhibition of isomerohydrolase activity (32% at 0.2 mM) in vitro
Bos taurus
3.1.1.64
2-(2-carboxyethyl)-2-methyl-4-phenyl-2H-imidazole-1-oxide
moderate inhibitory effect at 0.2 mM
Bos taurus
3.1.1.64
additional information
not inhibited by N-tert-butyl-(2-sulfophenyl)nitrone, alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone, NXY059, tert-butylhydroxylamine, and 2-methyl-2-nitrosopropane
Bos taurus
3.1.1.64
n-benzylidene-tert-butylamine
14% inhibition at 0.2 mM
Bos taurus
3.1.1.64
N-tert-butyl-alpha-phenylnitrone
strong uncompetitive inhibition (52%) of isomerohydrolase activity in vitro at 0.2 mM
Bos taurus
3.1.1.64
nitrosobenzene
strong inhibition of isomerohydrolase activity in vitro at 0.2 mM
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.1.64
0.061
-
N-tert-butyl-alpha-phenylnitrone
pH and temperature not specified in the publication
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
microsome
-
Bos taurus
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelial cell
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
714267
Bos taurus
11-cis-retinol + a fatty acid
RPE65 does not specifically produce 11-cis-retinol only but also 13-cis-retinol
-
-
?