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Literature summary extracted from

  • Zhang, Y.; Stubbe, J.
    Bacillus subtilis class Ib ribonucleotide reductase is a dimanganese(III)-tyrosyl radical enzyme (2011), Biochemistry, 50, 5615-5623.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP activates Bacillus subtilis
1.17.4.1 dATP activates Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.1 expression of N-terminally tagged NrdE and NrdF, the RNR genes are organized within the nrdI-nrdE-nrdF-ymaB operon, recombinant expression of NrdI, NdE, NrdF, and YmaB in Escherichia coli. For overexpression, the entire operon is placed behind a Pspank(hy) promoter and integrated into the Bacillus subtilis genome at the amyE site. Removal of the tag from rNrdE and rNrdF, leaving three amino acids GSH, has little effect on activity Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Fe2+ the isolated recombinant NrdF contains a diferric-tyrosyl radical [Fe(III)2-Y.] cofactor Bacillus subtilis
1.17.4.1 Mg2+ class Ib ribonucleotide reductase is a dimanganese(III)-tyrosyl radical enzyme, with Tyr115. Subunit beta, NrdF, contains the metallo-cofactor, essential for the initiation of the reduction process Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Bacillus subtilis
-
-
-
1.17.4.1 Bacillus subtilis JH624
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 recombinant NrdI, NdE, NrdF, and YmaB from Escherichia coli. The absorption spectra of the purified NrdENrdF complex exhibited characteristics of a Mn(III)2-Y radical center with 2 Mn/beta2 and 0.5 Y radical/beta2 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 dCDP + DTT disulfide + H2O in the assays for the Fe- and Mn-loaded recombinant NrdF, a 10fold excess of recombinant NrdE is used, CDP is the substrate, ATP or dATP is the effector, and DTT is the reductant Bacillus subtilis CDP + DTT
-
 r
1.17.4.1 dCDP + DTT disulfide + H2O in the assays for the Fe- and Mn-loaded recombinant NrdF, a 10fold excess of recombinant NrdE is used, CDP is the substrate, ATP or dATP is the effector, and DTT is the reductant Bacillus subtilis JH624 CDP + DTT
-
 r
1.17.4.1 additional information in the presence of rNrdE, ATP, and CDP, Mn(III)2-Y* and Fe(III)2-Y* rNrdF generate dCDP at rates of 132 and 10 nmol min/mg, respectively Bacillus subtilis ?
-
 ?
1.17.4.1 additional information in the presence of rNrdE, ATP, and CDP, Mn(III)2-Y* and Fe(III)2-Y* rNrdF generate dCDP at rates of 132 and 10 nmol min/mg, respectively Bacillus subtilis JH624 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.1 dimer alphabeta, class Ib RNR is composed of two subunits alpha (NrdE) and beta (NrdF). Beta contains the metallo-cofactor, essential for the initiation of the reduction process Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 class Ib ribonucleotide reductase
-
 Bacillus subtilis
1.17.4.1 class Ib RNR
-
 Bacillus subtilis
1.17.4.1 NrdE
-
 Bacillus subtilis
1.17.4.1 NrdF
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7.6
-
 assay at Bacillus subtilis

Expression

EC Number Organism Comment Expression
1.17.4.1 Bacillus subtilis RNR genes are induced in cells grown in Luria-Bertani medium, with levels of NrdE and NrdF elevated 35fold relative to that of the wild-type strain up

General Information

EC Number General Information Comment Organism
1.17.4.1 physiological function RNRs catalyze the conversion of nucleotides UDP, ADP, GDP, and CDP to deoxynucleotides, providing the monomeric building blocks required for DNA replication and repair Bacillus subtilis