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Literature summary extracted from

  • Bhattacharjee, H.; Sheng, J.; Ajees, A.; Mukhopadhyay, R.; Rosen, B.
    Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B and Cdc25C phosphatases (2010), Biochemistry, 49, 802-809.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.99.1 catalytic domain is expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.20.99.1 C120A inactive mutant Homo sapiens
1.20.99.1 additional information catalytic domain of Cdc25B is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals. Alteration of either the cysteine and arginine residues of the Cys-X5-Arg motif leads to the loss of both reductase and phosphatase activities Homo sapiens
1.20.99.1 R126A inactive mutant Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.99.1 0.9
-
arsenate pH 8.5, 30°C, 5 mM glutathione Homo sapiens
1.20.99.1 1.7
-
arsenate pH 8.5, 30°C, 2 mM glutathione Homo sapiens
1.20.99.1 2.5
-
arsenate pH 8.5, 30°C, 5 mM glutathione Homo sapiens
1.20.99.1 5
-
arsenate pH 8.5, 30°C, 2 mM glutathione Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.20.99.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.99.1 using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.99.1 arsenate + reduced acceptor in the presence of glutathione, human glutaredoxin, and human glutathione reductase, Cdc25B is found to catalyze the reduction of As(V) to As(III) Homo sapiens arsenite + acceptor
-
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Synonyms

EC Number Synonyms Comment Organism
1.20.99.1 Cdc25B isoform of human Cdc25 phosphatase is homologous to eukaryotic arsenate reductase and possesses arsenate reductase activity Homo sapiens
1.20.99.1 Cdc25C isoform of human Cdc25 phosphatase is homologous to eukaryotic arsenate reductase and possesses arsenate reductase activity Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.20.99.1 30
-
assay at and optimal temperature Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.20.99.1 0.012
-
arsenate pH 8.5, 30°C, 2 mM glutathione Homo sapiens
1.20.99.1 0.017
-
arsenate pH 8.5, 30°C, 2 mM glutathione Homo sapiens
1.20.99.1 0.025
-
arsenate pH 8.5, 30°C, 5 mM glutathione Homo sapiens
1.20.99.1 0.03
-
arsenate pH 8.5, 30°C, 5 mM glutathione Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.20.99.1 8.5
-
-
Homo sapiens

Expression

EC Number Organism Comment Expression
1.20.99.1 Homo sapiens catalytic domain of Cdc25C is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals additional information

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.20.99.1 0.0034
-
arsenate pH 8.5, 30°C, 2 mM glutathione Homo sapiens
1.20.99.1 0.007
-
arsenate pH 8.5, 30°C, 2 mM glutathione Homo sapiens
1.20.99.1 0.012
-
arsenate pH 8.5, 30°C, 5 mM glutathione Homo sapiens
1.20.99.1 0.027
-
arsenate pH 8.5, 30°C, 5 mM glutathione Homo sapiens