EC Number | Cloned (Comment) | Organism |
---|---|---|
1.20.99.1 | catalytic domain is expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.20.99.1 | C120A | inactive mutant | Homo sapiens |
1.20.99.1 | additional information | catalytic domain of Cdc25B is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals. Alteration of either the cysteine and arginine residues of the Cys-X5-Arg motif leads to the loss of both reductase and phosphatase activities | Homo sapiens |
1.20.99.1 | R126A | inactive mutant | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.99.1 | 0.9 | - |
arsenate | pH 8.5, 30°C, 5 mM glutathione | Homo sapiens | |
1.20.99.1 | 1.7 | - |
arsenate | pH 8.5, 30°C, 2 mM glutathione | Homo sapiens | |
1.20.99.1 | 2.5 | - |
arsenate | pH 8.5, 30°C, 5 mM glutathione | Homo sapiens | |
1.20.99.1 | 5 | - |
arsenate | pH 8.5, 30°C, 2 mM glutathione | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.20.99.1 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.20.99.1 | using Ni-NTA chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.20.99.1 | arsenate + reduced acceptor | in the presence of glutathione, human glutaredoxin, and human glutathione reductase, Cdc25B is found to catalyze the reduction of As(V) to As(III) | Homo sapiens | arsenite + acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.20.99.1 | Cdc25B | isoform of human Cdc25 phosphatase is homologous to eukaryotic arsenate reductase and possesses arsenate reductase activity | Homo sapiens |
1.20.99.1 | Cdc25C | isoform of human Cdc25 phosphatase is homologous to eukaryotic arsenate reductase and possesses arsenate reductase activity | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.20.99.1 | 30 | - |
assay at and optimal temperature | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.99.1 | 0.012 | - |
arsenate | pH 8.5, 30°C, 2 mM glutathione | Homo sapiens | |
1.20.99.1 | 0.017 | - |
arsenate | pH 8.5, 30°C, 2 mM glutathione | Homo sapiens | |
1.20.99.1 | 0.025 | - |
arsenate | pH 8.5, 30°C, 5 mM glutathione | Homo sapiens | |
1.20.99.1 | 0.03 | - |
arsenate | pH 8.5, 30°C, 5 mM glutathione | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.20.99.1 | 8.5 | - |
- |
Homo sapiens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.20.99.1 | Homo sapiens | catalytic domain of Cdc25C is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals | additional information |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.99.1 | 0.0034 | - |
arsenate | pH 8.5, 30°C, 2 mM glutathione | Homo sapiens | |
1.20.99.1 | 0.007 | - |
arsenate | pH 8.5, 30°C, 2 mM glutathione | Homo sapiens | |
1.20.99.1 | 0.012 | - |
arsenate | pH 8.5, 30°C, 5 mM glutathione | Homo sapiens | |
1.20.99.1 | 0.027 | - |
arsenate | pH 8.5, 30°C, 5 mM glutathione | Homo sapiens |