BRENDA - Enzyme Database show

Binding to lipid membrane induces conformational changes in RPE65: implications for its isomerohydrolase activity

Nikolaeva, O.; Moiseyev, G.; Rodgers, K.K.; Ma, J.X.; Biochem. J. 436, 591-597 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.64
expressed in HEK-293-LRAT cells
Gallus gallus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
membrane
membrane association of RPE65 is essential for its catalytic activity. The RPE65 membrane-binding affinity is significantly facilitated by incorporation of its substrate all-trans retinyl ester into liposomal membrane
Gallus gallus
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.64
Ni-NTA agarose column chromatography, gel filtration
Gallus gallus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
714103
Gallus gallus
11-cis-retinol + a fatty acid
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
expressed in HEK-293-LRAT cells
Gallus gallus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
membrane
membrane association of RPE65 is essential for its catalytic activity. The RPE65 membrane-binding affinity is significantly facilitated by incorporation of its substrate all-trans retinyl ester into liposomal membrane
Gallus gallus
16020
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
Ni-NTA agarose column chromatography, gel filtration
Gallus gallus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
714103
Gallus gallus
11-cis-retinol + a fatty acid
-
-
-
?