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Literature summary extracted from

  • Singh, R.; Wiseman, B.; Deemagarn, T.; Jha, V.; Switala, J.; Loewen, P.
    Comparative study of catalase-peroxidases (KatGs) (2008), Arch. Biochem. Biophys., 471, 207-214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli Escherichia coli
1.11.1.21 expressed in Escherichia coli Geobacillus stearothermophilus
1.11.1.21 expressed in Escherichia coli Rhodobacter capsulatus
1.11.1.21 expressed in Escherichia coli Mycobacterium tuberculosis
1.11.1.21 expressed in Escherichia coli Synechocystis sp.
1.11.1.21 expressed in Escherichia coli Archaeoglobus fulgidus
1.11.1.21 expressed in Escherichia coli Burkholderia pseudomallei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.21 azide highly sensitive Archaeoglobus fulgidus
1.11.1.21 azide highly sensitive Burkholderia pseudomallei
1.11.1.21 azide highly sensitive Escherichia coli
1.11.1.21 azide highly sensitive Geobacillus stearothermophilus
1.11.1.21 azide highly sensitive Mycobacterium tuberculosis
1.11.1.21 azide highly sensitive Rhodobacter capsulatus
1.11.1.21 azide highly sensitive Synechocystis sp.
1.11.1.21 cyanide highly sensitive Archaeoglobus fulgidus
1.11.1.21 cyanide highly sensitive Burkholderia pseudomallei
1.11.1.21 cyanide highly sensitive Escherichia coli
1.11.1.21 cyanide highly sensitive Geobacillus stearothermophilus
1.11.1.21 cyanide highly sensitive Mycobacterium tuberculosis
1.11.1.21 cyanide highly sensitive Rhodobacter capsulatus
1.11.1.21 cyanide highly sensitive Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 0.007
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Synechocystis sp.
1.11.1.21 0.016
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Archaeoglobus fulgidus
1.11.1.21 0.016
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 5.0 and 25°C Rhodobacter capsulatus
1.11.1.21 0.024
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Escherichia coli
1.11.1.21 0.031
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.0 and 25°C Geobacillus stearothermophilus
1.11.1.21 0.06
-
 H2O2 peroxidase reaction, at pH 4.3 and 25°C Escherichia coli
1.11.1.21 0.067
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.8 and 25°C Mycobacterium tuberculosis
1.11.1.21 0.095
-
 H2O2 peroxidase reaction, at pH 4.5 and 25°C Archaeoglobus fulgidus
1.11.1.21 0.21
-
 H2O2 peroxidase reaction, at pH 4.0 and 25°C Geobacillus stearothermophilus
1.11.1.21 0.3
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Burkholderia pseudomallei
1.11.1.21 0.36
-
 H2O2 peroxidase reaction, at pH 4.8 and 25°C Mycobacterium tuberculosis
1.11.1.21 0.7
-
 H2O2 peroxidase reaction, at pH 4.5 and 25°C Burkholderia pseudomallei
1.11.1.21 0.83
-
 H2O2 peroxidase reaction, at pH 5.0 and 25°C Rhodobacter capsulatus
1.11.1.21 1
-
 H2O2 peroxidase reaction, at pH 4.3 and 25°C Synechocystis sp.
1.11.1.21 2.4
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Mycobacterium tuberculosis
1.11.1.21 3.1
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Synechocystis sp.
1.11.1.21 3.7
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Geobacillus stearothermophilus
1.11.1.21 3.7
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Rhodobacter capsulatus
1.11.1.21 3.8
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Archaeoglobus fulgidus
1.11.1.21 4.2
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 4.5
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Burkholderia pseudomallei
1.11.1.21 20
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Synechocystis sp.
1.11.1.21 30
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Rhodobacter capsulatus
1.11.1.21 32
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Archaeoglobus fulgidus
1.11.1.21 35
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Escherichia coli
1.11.1.21 56
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Burkholderia pseudomallei
1.11.1.21 90
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Geobacillus stearothermophilus
1.11.1.21 225
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.21 80000
-
 about 80000, SDS-PAGE Escherichia coli
1.11.1.21 80000
-
 about 80000, SDS-PAGE Geobacillus stearothermophilus
1.11.1.21 80000
-
 about 80000, SDS-PAGE Rhodobacter capsulatus
1.11.1.21 80000
-
 about 80000, SDS-PAGE Mycobacterium tuberculosis
1.11.1.21 80000
-
 about 80000, SDS-PAGE Synechocystis sp.
1.11.1.21 80000
-
 about 80000, SDS-PAGE Archaeoglobus fulgidus
1.11.1.21 80000
-
 about 80000, SDS-PAGE Burkholderia pseudomallei

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Archaeoglobus fulgidus
-
-
-
1.11.1.21 Burkholderia pseudomallei
-
-
-
1.11.1.21 Escherichia coli
-
-
-
1.11.1.21 Geobacillus stearothermophilus
-
-
-
1.11.1.21 Mycobacterium tuberculosis
-
-
-
1.11.1.21 Rhodobacter capsulatus
-
-
-
1.11.1.21 Synechocystis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 ammonium sulfate precipitation and DEAE-cellulose column chromatography Escherichia coli
1.11.1.21 ammonium sulfate precipitation and DEAE-cellulose column chromatography Geobacillus stearothermophilus
1.11.1.21 ammonium sulfate precipitation and DEAE-cellulose column chromatography Rhodobacter capsulatus
1.11.1.21 ammonium sulfate precipitation and DEAE-cellulose column chromatography Mycobacterium tuberculosis
1.11.1.21 ammonium sulfate precipitation and DEAE-cellulose column chromatography Synechocystis sp.
1.11.1.21 ammonium sulfate precipitation and DEAE-cellulose column chromatography Archaeoglobus fulgidus
1.11.1.21 ammonium sulfate precipitation and DEAE-cellulose column chromatography Burkholderia pseudomallei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 1
-
 peroxidase specific activity, using o-dianisidine as substrate, at pH 5.0 and 25°C Rhodobacter capsulatus
1.11.1.21 2
-
 peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 5.0 and 25°C Rhodobacter capsulatus
1.11.1.21 3.2
-
 peroxidase specific activity, using o-dianisidine as substrate, at pH 4.5 and 25°C Archaeoglobus fulgidus
1.11.1.21 4.8
-
 peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C Burkholderia pseudomallei
1.11.1.21 5.3
-
 peroxidase specific activity, using o-dianisidine as substrate, at pH 4.0 and 25°C Geobacillus stearothermophilus
1.11.1.21 5.3
-
 peroxidase specific activity, using o-dianisidine as substrate, at pH 4.5 and 25°C Burkholderia pseudomallei
1.11.1.21 5.5
-
 peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C Synechocystis sp.
1.11.1.21 5.9
-
 peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.0 and 25°C Geobacillus stearothermophilus
1.11.1.21 6.4
-
 peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.3 and 25°C Synechocystis sp.
1.11.1.21 8.3
-
 peroxidase specific activity, using o-dianisidine as substrate, at pH 4.3 and 25°C Escherichia coli
1.11.1.21 8.8
-
 peroxidase specific activity, using o-dianisidine as substrate, at pH 4.8 and 25°C Mycobacterium tuberculosis
1.11.1.21 10
-
 peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.8 and 25°C Mycobacterium tuberculosis
1.11.1.21 11
-
 peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.3 and 25°C Escherichia coli
1.11.1.21 12
-
 peroxidase specific activity, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 4.5 and 25°C Archaeoglobus fulgidus
1.11.1.21 2200
-
 catalase specific activity, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 3120
-
 catalase specific activity, at pH 7.0 and 37°C Geobacillus stearothermophilus
1.11.1.21 3630
-
 catalase specific activity, at pH 7.0 and 37°C Burkholderia pseudomallei
1.11.1.21 4450
-
 catalase specific activity, at pH 7.0 and 37°C Mycobacterium tuberculosis
1.11.1.21 4830
-
 catalase specific activity, at pH 7.0 and 37°C Rhodobacter capsulatus
1.11.1.21 5280
-
 catalase specific activity, at pH 7.0 and 37°C Archaeoglobus fulgidus
1.11.1.21 5420
-
 catalase specific activity, at pH 7.0 and 37°C Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Geobacillus stearothermophilus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Rhodobacter capsulatus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Mycobacterium tuberculosis oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Synechocystis sp. oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Archaeoglobus fulgidus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Burkholderia pseudomallei oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.21 H2O2
-
 Escherichia coli O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Geobacillus stearothermophilus O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Rhodobacter capsulatus O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Synechocystis sp. O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Archaeoglobus fulgidus O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Burkholderia pseudomallei O2 + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Escherichia coli oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Geobacillus stearothermophilus oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Rhodobacter capsulatus oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Mycobacterium tuberculosis oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Synechocystis sp. oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Archaeoglobus fulgidus oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Burkholderia pseudomallei oxidized o-dianisidine + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Escherichia coli
1.11.1.21 KatG
-
 Geobacillus stearothermophilus
1.11.1.21 KatG
-
 Rhodobacter capsulatus
1.11.1.21 KatG
-
 Mycobacterium tuberculosis
1.11.1.21 KatG
-
 Synechocystis sp.
1.11.1.21 KatG
-
 Archaeoglobus fulgidus
1.11.1.21 KatG
-
 Burkholderia pseudomallei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.21 65
-
 the enzyme is completely inactive after less than 1 min at 65°C Rhodobacter capsulatus
1.11.1.21 65
-
 the enzyme is completely inactive after less than 1 min at 65°C Synechocystis sp.
1.11.1.21 65
-
 the enzyme is completely inactive after less than 1 min at 65°C Burkholderia pseudomallei
1.11.1.21 65
-
 the enzyme is completely inactive after less than 1.5 min at 65°C Escherichia coli
1.11.1.21 65
-
 the enzyme is completely inactive after less than 1.5 min at 65°C Mycobacterium tuberculosis
1.11.1.21 65
-
 the enzyme is completely inactive after more than 40 min at 65°C Geobacillus stearothermophilus
1.11.1.21 65
-
 the enzyme is completely inactive after more than 40 min at 65°C Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 7.7
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 5.0 and 25°C Rhodobacter capsulatus
1.11.1.21 7.9
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Burkholderia pseudomallei
1.11.1.21 11
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.0 and 25°C Geobacillus stearothermophilus
1.11.1.21 13
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Synechocystis sp.
1.11.1.21 17
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.5 and 25°C Archaeoglobus fulgidus
1.11.1.21 19
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.8 and 25°C Mycobacterium tuberculosis
1.11.1.21 25
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) peroxidase reaction, at pH 4.3 and 25°C Escherichia coli
1.11.1.21 2950
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Escherichia coli
1.11.1.21 4300
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Geobacillus stearothermophilus
1.11.1.21 4350
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Mycobacterium tuberculosis
1.11.1.21 4970
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Escherichia coli
1.11.1.21 5680
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Burkholderia pseudomallei
1.11.1.21 6640
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Rhodobacter capsulatus
1.11.1.21 7600
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Geobacillus stearothermophilus
1.11.1.21 7630
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Synechocystis sp.
1.11.1.21 7770
-
 H2O2 catalase reaction, at pH 7.0 and 37°C Archaeoglobus fulgidus
1.11.1.21 8000
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Synechocystis sp.
1.11.1.21 10200
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Mycobacterium tuberculosis
1.11.1.21 14100
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Rhodobacter capsulatus
1.11.1.21 15680
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Archaeoglobus fulgidus
1.11.1.21 15900
-
 H2O2 catalase reaction, at pH 5.5-6.0 and 37°C Burkholderia pseudomallei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 4
-
-
 Geobacillus stearothermophilus
1.11.1.21 4.3
-
-
 Escherichia coli
1.11.1.21 4.3
-
-
 Synechocystis sp.
1.11.1.21 4.5
-
-
 Archaeoglobus fulgidus
1.11.1.21 4.5
-
-
 Burkholderia pseudomallei
1.11.1.21 4.8
-
-
 Mycobacterium tuberculosis
1.11.1.21 5
-
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Escherichia coli
1.11.1.21 heme
-
 Geobacillus stearothermophilus
1.11.1.21 heme
-
 Rhodobacter capsulatus
1.11.1.21 heme
-
 Mycobacterium tuberculosis
1.11.1.21 heme
-
 Synechocystis sp.
1.11.1.21 heme
-
 Archaeoglobus fulgidus
1.11.1.21 heme
-
 Burkholderia pseudomallei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.21 0.0002
-
 at pH 7.0 and 37°C Mycobacterium tuberculosis azide
1.11.1.21 0.002
-
 at pH 7.0 and 37°C Geobacillus stearothermophilus azide
1.11.1.21 0.007
-
 at pH 7.0 and 37°C Escherichia coli azide
1.11.1.21 0.008
-
 at pH 7.0 and 37°C Rhodobacter capsulatus azide
1.11.1.21 0.008
-
 at pH 7.0 and 37°C Burkholderia pseudomallei azide
1.11.1.21 0.013
-
 at pH 7.0 and 37°C Synechocystis sp. azide
1.11.1.21 0.02
-
 at pH 7.0 and 37°C Archaeoglobus fulgidus azide
1.11.1.21 0.04
-
 at pH 7.0 and 37°C Burkholderia pseudomallei cyanide
1.11.1.21 0.05
-
 at pH 7.0 and 37°C Escherichia coli cyanide
1.11.1.21 0.05
-
 at pH 7.0 and 37°C Geobacillus stearothermophilus cyanide
1.11.1.21 0.08
-
 at pH 7.0 and 37°C Rhodobacter capsulatus cyanide
1.11.1.21 0.1
-
 at pH 7.0 and 37°C Mycobacterium tuberculosis cyanide
1.11.1.21 0.15
-
 at pH 7.0 and 37°C Archaeoglobus fulgidus cyanide
1.11.1.21 0.17
-
 at pH 7.0 and 37°C Synechocystis sp. cyanide