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Literature summary extracted from

  • Kantz, A.; Chin, F.; Nallamothu, N.; Nguyen, T.; Gassner, G.T.
    Mechanism of flavin transfer and oxygen activation by the two-component flavoenzyme styrene monooxygenase (2005), Arch. Biochem. Biophys., 442, 102-116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.11 expression of native and histidine-tagged versions of SMOA in Escherichia coli BL21 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.11 Benzene competitive inhibitor of styrene Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.11 0.0046
-
 Styrene pH 7.0, 37°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2 Pseudomonas putida
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 Pseudomonas putida S12
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.11 Pseudomonas putida
-
-
-
1.14.14.11 Pseudomonas putida S12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.11
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas putida (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 coupling of NADH and styrene oxidation can be best explained by a model, which includes both the direct transfer and passive diffusion of reduced FAD from NADH-specific flavin reductase (SMOB) to FAD-specific styrene epoxidase (SMOA) Pseudomonas putida (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas putida S12 (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 coupling of NADH and styrene oxidation can be best explained by a model, which includes both the direct transfer and passive diffusion of reduced FAD from NADH-specific flavin reductase (SMOB) to FAD-specific styrene epoxidase (SMOA) Pseudomonas putida S12 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.11 FAD-specific styrene epoxidase
-
 Pseudomonas putida
1.14.14.11 SMO styrene monooxygenase (SMO) from Pseudomonas putida S12 is a two-component flavoenzyme composed of the NADH-specific flavin reductase, SMOB, and FAD-specific styrene epoxidase, SMOA Pseudomonas putida
1.14.14.11 SmoA styrene monooxygenase (SMO) from Pseudomonas putida S12 is a two-component flavoenzyme composed of the NADH-specific flavin reductase, SMOB, and FAD-specific styrene epoxidase, SMOA Pseudomonas putida