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Literature summary extracted from

  • Temuujin, U.; Chi, W.J.; Lee, S.Y.; Chang, Y.K.; Hong, S.K.
    Overexpression and biochemical characterization of DagA from Streptomyces coelicolor A3(2): an endo-type beta-agarase producing neoagarotetraose and neoagarohexaose (2011), Appl. Microbiol. Biotechnol., 92, 749-759.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 expressed in Streptomyces lividans strain TK24 Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 Cu2+ 79% inhibition at 1 mM Streptomyces coelicolor
3.2.1.81 EDTA the inhibition by 1 mM EDTA is almost completely recovered by the addition of Co2+ at a concentration of 0.2 mM Streptomyces coelicolor
3.2.1.81 EGTA the inhibition by 1 mM EGTA is almost completely recovered by the addition of Co2+ at a concentration of 0.2 mM Streptomyces coelicolor
3.2.1.81 Mn2+ 56% inhibition at 1 mM Streptomyces coelicolor
3.2.1.81 additional information EDTA and EGTA do not affect on the activity at a concentration of 0.2 mM Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.81 0.0182
-
 agarose in 50 mM sodium phosphate buffer, pH 7.0, 40°C Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.81 Co2+ 135% activity at 1 mM Streptomyces coelicolor
3.2.1.81 additional information Ca2+, Mg2+, Ni2+, or Zn2+ do not affect on the activity at a concentration of 0.2 mM Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 32000
-
 x * 32000, mature protein, SDS-PAGE Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Streptomyces coelicolor
-
-
-
3.2.1.81 Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81 agarose bead chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
 Streptomyces coelicolor neoagarotetraose + neoagarohexaose
-
 ?
3.2.1.81 agarose + H2O
-
 Streptomyces coelicolor A3(2) neoagarotetraose + neoagarohexaose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 ? x * 32000, mature protein, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 DagA an endo-type beta-agarase Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 40
-
-
 Streptomyces coelicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.81 25 40 the enzyme works efficiently in the range of 25-40°C and shows maximum activity at 40°C but loses essentially all of its activity at 60°C Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 20 45 heat treatment in the range of 20-40°C does not affect the residual activity of the enzyme. Only 45% of the activity is retained after heating at 50°C for 1 h Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 7
-
-
 Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.81 4 9 the enzyme shows more than 90% activity between pH 5.0 and 8.0. More than 50% of the agarase activity is lost below pH 4.0 and above pH 9.0 Streptomyces coelicolor