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Literature summary extracted from

  • Wang, G.; Meng, K.; Luo, H.; Wang, Y.; Huang, H.; Shi, P.; Pan, X.; Yang, P.; Yao, B.
    Molecular cloning and characterization of a novel SGNH arylesterase from the goat rumen contents (2011), Appl. Microbiol. Biotechnol., 91, 1561-1570.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.2 Nonidet P-40 8.2% activation at 5% (v/v) uncultured organism
3.1.1.2 Triton X-100 11.2% activation at 5% (v/v) uncultured organism
3.1.1.2 Tween 20 4.8% activation at 5% (v/v) uncultured organism
3.1.1.2 Tween 40 16.4% activation at 5% (v/v) uncultured organism
3.1.1.2 Tween 80 19.6% activation at 5% (v/v) uncultured organism

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.2 expressed in Escherichia coli BL21(DE3) cells uncultured organism

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 2-mercaptoethanol 73.5% residual activity at 5 mM uncultured organism
3.1.1.2 Ag+ 9.7% residual activity at 5 mM uncultured organism
3.1.1.2 Ca2+ 88.5% residual activity at 5 mM uncultured organism
3.1.1.2 Co2+ 92.6% residual activity at 5 mM uncultured organism
3.1.1.2 Cr3+ 39.8% residual activity at 5 mM uncultured organism
3.1.1.2 Cu2+ 28.9% residual activity at 5 mM uncultured organism
3.1.1.2 diethyl dicarbonate 5.5% residual activity at 5 mM uncultured organism
3.1.1.2 dithiothreitol 19.4% residual activity at 5 mM uncultured organism
3.1.1.2 EDTA 94.2% residual activity at 5 mM uncultured organism
3.1.1.2 Fe3+ 13.7% residual activity at 5 mM uncultured organism
3.1.1.2 Hg2+ 7.2% residual activity at 5 mM uncultured organism
3.1.1.2 Mg2+ 89.2% residual activity at 5 mM uncultured organism
3.1.1.2 Mn2+ 85.4% residual activity at 5 mM uncultured organism
3.1.1.2 additional information addition of glycerin has no effect on the enzyme activity uncultured organism
3.1.1.2 NaCl 43.7% residual activity at 4 M uncultured organism
3.1.1.2 Ni2+ 86.3% residual activity at 5 mM uncultured organism
3.1.1.2 Pb2+ 45.7% residual activity at 5 mM uncultured organism
3.1.1.2 phenylmethylsulfonyl fluoride complete inhibition at 5 mM uncultured organism
3.1.1.2 SDS complete inhibition at 5% (w/v) uncultured organism
3.1.1.2 Urea 64.7% residual activity at 8 M uncultured organism
3.1.1.2 Zn2+ 76.4% residual activity at 5 mM uncultured organism

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.041
-
1-naphthyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
3.1.1.2 0.53
-
phenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
3.1.1.2 0.96
-
4-nitrophenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 20000
-
x * 20000, SDS-PAGE uncultured organism
3.1.1.2 24000
-
x * 24000, calculated from amino acid sequence uncultured organism

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 uncultured organism G0YFD9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 75 gel filtration uncultured organism

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 additional information bacterium from goat rumen content uncultured organism
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 1-naphthyl acetate + H2O 15.4% activity compared to 4-nitrophenyl acetate uncultured organism 1-naphthol + acetate
-
?
3.1.1.2 4-nitrophenyl acetate + H2O highest catalytic efficiency (100% activity) uncultured organism 4-nitrophenol + acetate
-
?
3.1.1.2 4-nitrophenyl butyrate + H2O 19.7% activity compared to 4-nitrophenyl acetate uncultured organism 4-nitrophenol + butyrate
-
?
3.1.1.2 4-nitrophenyl caproate + H2O 4.1% activity compared to 4-nitrophenyl acetate uncultured organism 4-nitrophenol + caproate
-
?
3.1.1.2 additional information the enzyme has no activity towards 4-nitrophenyl esters with acyl chains longer than C8 like 4-nitrophenyl caprylate, 4-nitrophenyl caprate, 4-nitrophenyl palmitate, as well as 4-nitrophenyl phosphate and methyl parathion uncultured organism ?
-
?
3.1.1.2 phenyl acetate + H2O 166% activity compared to 4-nitrophenyl acetate uncultured organism phenol + acetate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 ? x * 20000, SDS-PAGE uncultured organism
3.1.1.2 ? x * 24000, calculated from amino acid sequence uncultured organism

Synonyms

EC Number Synonyms Comment Organism
3.1.1.2 EstR5
-
uncultured organism
3.1.1.2 SGNH arylesterase
-
uncultured organism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 60
-
-
uncultured organism

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.2 40 65 the enzyme exhibits more than 50% of the maximal activity between 40°C and 65°C uncultured organism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.2 45 60 the enzyme is stable at 45°C, retaining 91.5% of the activity after 1 h incubation. At 60°C, the enzyme activity decreases rapidly and only retains 16.8% of the activity after 1 h incubation uncultured organism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 8
-
-
uncultured organism

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.2 6 9 more than 60% of the maximum activity is retained between pH 6.0 and 9.0 uncultured organism

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.2 5 11 the enzyme is stable between pH 5.0 and 11.0, retaining more than 50% of the initial activity after incubation at 37°C for 1 h uncultured organism

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.2 uncultured organism calculated from amino acid sequence
-
4.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.2 204.5
-
4-nitrophenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
3.1.1.2 401.4
-
phenyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism
3.1.1.2 1612
-
1-naphthyl acetate in 50 mM Tris-HCl (pH 8.0), at 37°C uncultured organism