EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.2 | Nonidet P-40 | 8.2% activation at 5% (v/v) | uncultured organism | |
3.1.1.2 | Triton X-100 | 11.2% activation at 5% (v/v) | uncultured organism | |
3.1.1.2 | Tween 20 | 4.8% activation at 5% (v/v) | uncultured organism | |
3.1.1.2 | Tween 40 | 16.4% activation at 5% (v/v) | uncultured organism | |
3.1.1.2 | Tween 80 | 19.6% activation at 5% (v/v) | uncultured organism |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.2 | expressed in Escherichia coli BL21(DE3) cells | uncultured organism |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.2 | 2-mercaptoethanol | 73.5% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Ag+ | 9.7% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Ca2+ | 88.5% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Co2+ | 92.6% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Cr3+ | 39.8% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Cu2+ | 28.9% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | diethyl dicarbonate | 5.5% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | dithiothreitol | 19.4% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | EDTA | 94.2% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Fe3+ | 13.7% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Hg2+ | 7.2% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Mg2+ | 89.2% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Mn2+ | 85.4% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | additional information | addition of glycerin has no effect on the enzyme activity | uncultured organism | |
3.1.1.2 | NaCl | 43.7% residual activity at 4 M | uncultured organism | |
3.1.1.2 | Ni2+ | 86.3% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | Pb2+ | 45.7% residual activity at 5 mM | uncultured organism | |
3.1.1.2 | phenylmethylsulfonyl fluoride | complete inhibition at 5 mM | uncultured organism | |
3.1.1.2 | SDS | complete inhibition at 5% (w/v) | uncultured organism | |
3.1.1.2 | Urea | 64.7% residual activity at 8 M | uncultured organism | |
3.1.1.2 | Zn2+ | 76.4% residual activity at 5 mM | uncultured organism |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 0.041 | - |
1-naphthyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
3.1.1.2 | 0.53 | - |
phenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
3.1.1.2 | 0.96 | - |
4-nitrophenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 20000 | - |
x * 20000, SDS-PAGE | uncultured organism |
3.1.1.2 | 24000 | - |
x * 24000, calculated from amino acid sequence | uncultured organism |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.2 | uncultured organism | G0YFD9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.2 | ammonium sulfate precipitation, Q Sepharose column chromatography, and Superdex 75 gel filtration | uncultured organism |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.2 | additional information | bacterium from goat rumen content | uncultured organism | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.2 | 1-naphthyl acetate + H2O | 15.4% activity compared to 4-nitrophenyl acetate | uncultured organism | 1-naphthol + acetate | - |
? | |
3.1.1.2 | 4-nitrophenyl acetate + H2O | highest catalytic efficiency (100% activity) | uncultured organism | 4-nitrophenol + acetate | - |
? | |
3.1.1.2 | 4-nitrophenyl butyrate + H2O | 19.7% activity compared to 4-nitrophenyl acetate | uncultured organism | 4-nitrophenol + butyrate | - |
? | |
3.1.1.2 | 4-nitrophenyl caproate + H2O | 4.1% activity compared to 4-nitrophenyl acetate | uncultured organism | 4-nitrophenol + caproate | - |
? | |
3.1.1.2 | additional information | the enzyme has no activity towards 4-nitrophenyl esters with acyl chains longer than C8 like 4-nitrophenyl caprylate, 4-nitrophenyl caprate, 4-nitrophenyl palmitate, as well as 4-nitrophenyl phosphate and methyl parathion | uncultured organism | ? | - |
? | |
3.1.1.2 | phenyl acetate + H2O | 166% activity compared to 4-nitrophenyl acetate | uncultured organism | phenol + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.2 | ? | x * 20000, SDS-PAGE | uncultured organism |
3.1.1.2 | ? | x * 24000, calculated from amino acid sequence | uncultured organism |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.2 | EstR5 | - |
uncultured organism |
3.1.1.2 | SGNH arylesterase | - |
uncultured organism |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 60 | - |
- |
uncultured organism |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 40 | 65 | the enzyme exhibits more than 50% of the maximal activity between 40°C and 65°C | uncultured organism |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 45 | 60 | the enzyme is stable at 45°C, retaining 91.5% of the activity after 1 h incubation. At 60°C, the enzyme activity decreases rapidly and only retains 16.8% of the activity after 1 h incubation | uncultured organism |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 8 | - |
- |
uncultured organism |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 6 | 9 | more than 60% of the maximum activity is retained between pH 6.0 and 9.0 | uncultured organism |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.2 | 5 | 11 | the enzyme is stable between pH 5.0 and 11.0, retaining more than 50% of the initial activity after incubation at 37°C for 1 h | uncultured organism |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.2 | uncultured organism | calculated from amino acid sequence | - |
4.6 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 204.5 | - |
4-nitrophenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
3.1.1.2 | 401.4 | - |
phenyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism | |
3.1.1.2 | 1612 | - |
1-naphthyl acetate | in 50 mM Tris-HCl (pH 8.0), at 37°C | uncultured organism |