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Literature summary extracted from

  • OConnell, E.; Piggott, C.; Tuohy, M.
    Purification of exo-1,3-beta-glucanase, a new extracellular glucanolytic enzyme from Talaromyces emersonii (2011), Appl. Microbiol. Biotechnol., 89, 685-696.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 glucono-delta-lactone
-
 Rasamsonia emersonii
3.2.1.58 KMnO4 10 mM, 60% residual activity Rasamsonia emersonii
3.2.1.58 L-cysteine 10 mM, 68% residual activity Rasamsonia emersonii
3.2.1.58 N-bromosuccinimide 10 mM, 0.1% residual activity Rasamsonia emersonii
3.2.1.58 phenylglyoxal hydrate 10 mM, 86% residual activity Rasamsonia emersonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 additional information
-
 Laminaran KM-value is 1.66 mg/ml Rasamsonia emersonii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.58 extracellular
-
 Rasamsonia emersonii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.58 Cu2+ 10 mM, 82% residual activity Rasamsonia emersonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 40000
-
 PAGE Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Rasamsonia emersonii
-
 member of glycosyl hydrolase family 5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58
-
 Rasamsonia emersonii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 592
-
 pH 5.0, 50°C Rasamsonia emersonii

Storage Stability

EC Number Storage Stability Organism
3.2.1.58 4°C, pH 5.0, stable for at least 6 months Rasamsonia emersonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 laminaran + H2O at pH 5.0, strictly specific for laminaran and laminarioligosaccharides, with a strict exo mode of action, with glucose being the sole reaction product even at the initial stages of hydrolysis Rasamsonia emersonii D-glucose + ?
-
 ?
3.2.1.58 laminaripentaose + H2O 118% of the activity with laminaran Rasamsonia emersonii D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
 ?
3.2.1.58 laminaritetraose + H2O 121% of the activity with laminaran Rasamsonia emersonii D-glucose + laminaribiose + laminaritriose
-
 ?
3.2.1.58 laminaritriose + H2O 115% of the activity with laminaran Rasamsonia emersonii D-glucose + laminaribiose
-
 ?
3.2.1.58 additional information no substrate: beta-1,4-glucans, beta-1,3/1,4-glucans or 4-nitrophenyl- and methylumbelliferyl-beta-D-glucopyranosides, carboxymethyl cellulose, avicel. Trace activity with pustulan Rasamsonia emersonii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 40000, SDS-PAGE Rasamsonia emersonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 65
-
-
 Rasamsonia emersonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 65
-
 half-life 18.3 h Rasamsonia emersonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.58 29550
-
 Laminaran pH 5.0, 50°C Rasamsonia emersonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 5.4
-
-
 Rasamsonia emersonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.58 2.8 8 more than 50% of maximum activity within Rasamsonia emersonii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.58 1.25
-
 glucono-delta-lactone pH 5.0, 50°C Rasamsonia emersonii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.58 Rasamsonia emersonii isoelectric focusing
-
 4.3