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Literature summary extracted from

  • Kamennaya, N.A.; Post, A.F.
    Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp (2011), Appl. Environ. Microbiol., 77, 291-301.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.104 gene cynS, cynS is presumably transcribed as part of the cynABDS operon, DNA and amino acid sequence determination and analysis, phylogenetic analysis Prochlorococcus sp.
4.2.1.104 gene cynS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in Escherichia coli strain HMS174 as maltose-binding-protein fusion protein Synechococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.104 Na-azide
-
 Prochlorococcus sp.
4.2.1.104 Na-azide
-
 Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.104 50000
-
 x * 50000, recombinant MBP-CynS fusion enzyme, SDS-PAGE Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.104 cyanate + HCO3- + 2 H+ Synechococcus sp.
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Prochlorococcus sp.
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Prochlorococcus sp. MED4
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Synechococcus sp. WH7803
-
 NH3 + 2 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.104 Prochlorococcus sp. Q7V2U0 gene cynS
-
4.2.1.104 Prochlorococcus sp. MED4 Q7V2U0 gene cynS
-
4.2.1.104 Synechococcus sp. A5GPQ6 gene cynS
-
4.2.1.104 Synechococcus sp. WH7803 A5GPQ6 gene cynS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.104 recombinant CynS-MBP from Escherichia coli strain HMS174 by amylose affinity chromatography Synechococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.104 0.43
-
 purified recombinant enzyme, pH 7.4, 55°C Synechococcus sp.
4.2.1.104 0.8
-
 purified recombinant enzyme, pH 7.4, 4°C Synechococcus sp.
4.2.1.104 5.56
-
 purified recombinant enzyme, pH 7.4, 26°C Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Synechococcus sp. NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Prochlorococcus sp. NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Prochlorococcus sp. MED4 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Synechococcus sp. WH7803 NH3 + 2 CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.104 ? x * 50000, recombinant MBP-CynS fusion enzyme, SDS-PAGE Synechococcus sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.1.104 cyanate lyase
-
 Synechococcus sp.
4.2.1.104 CynS
-
 Prochlorococcus sp.
4.2.1.104 CynS
-
 Synechococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.104 26
-
-
 Synechococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.104 4 55 activity range Synechococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.104 4 55 CynS is rapidly inactivated at higher temperatures and only residual activity o f 0.09 U/mg occurs at 50°C Synechococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.104 7.4
-
 assay at Synechococcus sp.

General Information

EC Number General Information Comment Organism
4.2.1.104 additional information transcriptional regulation of cynS expression, overview Synechococcus sp.
4.2.1.104 additional information transcriptional regulation of cynS expression, overview Prochlorococcus sp.