BRENDA - Enzyme Database show

Identification of an aox system that requires cytochrome c in the highly arsenic-resistant bacterium Ochrobactrum tritici SCII24

Branco, R.; Francisco, R.; Chung, A.; Morais, P.; Appl. Environ. Microbiol. 75, 5141-5147 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.20.2.1
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer; functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.20.2.1
additional information
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer; functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.2.1
additional information
no activity detected in membrane fraction; no activity detected in membrane fraction
Ochrobactrum tritici
-
-
1.20.2.1
soluble
;
Ochrobactrum tritici
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.2.1
Ochrobactrum tritici
B7UB93
small subunit aoxA
-
1.20.2.1
Ochrobactrum tritici
B7UB94
large subunit aoxB
-
1.20.2.1
Ochrobactrum tritici SCII24
B7UB93
small subunit aoxA
-
1.20.2.1
Ochrobactrum tritici SCII24
B7UB94
large subunit aoxB
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici SCII24
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.2.1
6
-
;
Ochrobactrum tritici
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.2.1
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.20.2.1
additional information
functional expression in Ochrobacterium tritici 5bvl1, a non-As(III) oxidizer
Ochrobactrum tritici
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.2.1
additional information
no activity detected in membrane fraction
Ochrobactrum tritici
-
-
1.20.2.1
soluble
-
Ochrobactrum tritici
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.20.2.1
arsenite + H2O + 2,6-dichlorophenolindophenol
-
713822
Ochrobactrum tritici SCII24
arsenate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.2.1
6
-
-
Ochrobactrum tritici
Expression
EC Number
Organism
Commentary
Expression
1.20.2.1
Ochrobactrum tritici
coexpression of genes aoxA, aoxB and cytC independent of the remaining genes of the operon, aoxS and aoxR; coexpression of genes aoxA, aoxB and cytC independent of the remaining genes of the operon, aoxS and aoxR
additional information
General Information
EC Number
General Information
Commentary
Organism
1.20.2.1
physiological function
the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon; the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon. An aoxB deletion mutant fails to oxidize As(III) to As(V), but is able to grow in the presence of high arsenite concentrations
Ochrobactrum tritici
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.20.2.1
physiological function
the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon. An aoxB deletion mutant fails to oxidize As(III) to As(V), but is able to grow in the presence of high arsenite concentrations
Ochrobactrum tritici
1.20.2.1
physiological function
the As(III) oxidation process in Ochrobacterium tritici requires not only the enzyme arsenite oxidase but also the cytochrome c encoded in the operon
Ochrobactrum tritici
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.20.2.1
Ochrobactrum tritici
coexpression of genes aoxA, aoxB and cytC independent of the remaining genes of the operon, aoxS and aoxR
additional information