EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.25 | synthesis | the recombinant enzyme is a highly efficient biocatalyst for asymmetric synthesis of chiral compounds | Fusarium proliferatum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.25 | expressed in Escherichia coli JM109(DE3) cells | Fusarium proliferatum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.25 | Ag+ | 33.2% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Al3+ | 22.4% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Co2+ | 10.7% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Cu+ | 13.6% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Cu2+ | 11.2% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Fe2+ | 15.6% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Fe3+ | 3.3% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Hg2+ | 2.1% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Li+ | 24.7% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Mg2+ | 32.4% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Mn2+ | 22.7% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | additional information | EDTA does not exhibit significant effect on enzyme activity | Fusarium proliferatum | |
3.1.1.25 | Ni2+ | 55.4% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Pb2+ | 13.9% residual activity at 1 mM | Fusarium proliferatum | |
3.1.1.25 | Zn2+ | 14.3% residual activity at 1 mM | Fusarium proliferatum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.25 | 121 | - |
DL-pantoyllactone | recombinant enzyme, at pH 6.7-6.9, 30°C | Fusarium proliferatum | |
3.1.1.25 | 290.2 | - |
2-hydroxybutyrolactone | recombinant enzyme, at pH 6.7-6.9, 30°C | Fusarium proliferatum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.25 | Ca2+ | 7.4% activation at 1 mM. No significant effect of Ca2+ on the stability of recombinant enzyme is observed when incubated together for 60 h at 30°C or 4°C | Fusarium proliferatum | |
3.1.1.25 | additional information | the enzyme is not affected by Na+ and K+ | Fusarium proliferatum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.25 | 50000 | - |
x * 50000, recombinant protein, SDS-PAGE | Fusarium proliferatum |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.25 | Acetone | 49% residual activity at 10% (v/v), when the concentration of methanol is increased up to 20% (v/v), up to 30% of the initial activity is retained | Fusarium proliferatum |
3.1.1.25 | acetonitrile | 55% residual activity at 10% (v/v), when the concentration of methanol is increased up to 20% (v/v), up to 30% of the initial activity is retained | Fusarium proliferatum |
3.1.1.25 | dimethylformamide | 51% residual activity at 10% (v/v) | Fusarium proliferatum |
3.1.1.25 | Ethanol | 55% residual activity at 10% (v/v) | Fusarium proliferatum |
3.1.1.25 | Methanol | 81% residual activity at 10% (v/v), when the concentration of methanol is increased up to 20% (v/v), 60.5% of the initial activity is retained | Fusarium proliferatum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.25 | Fusarium proliferatum | B2LYJ5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.25 | Ni-NTA column chromatography | Fusarium proliferatum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.25 | 341.2 | - |
recombinant enzyme after 14.4fold purification, using DL-pantoyl lactone as substrate, at pH 6.7-6.9, 30°C | Fusarium proliferatum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.25 | 2-bromobutyrolactone + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | 2-hydroxybutyrolactone + H2O | 2450% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | 4-butyrolactone + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | 4-phenyl-2-hydroxyl-4-butyrolactone + H2O | 22% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | DL-pantoyllactone + H2O | 100% activity | Fusarium proliferatum | pantoic acid | - |
? | |
3.1.1.25 | ethyl acetate + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | methyl 2-chloroacetate + H2O | 11.6% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | methyl 2-chloropropanoate + H2O | 2.3% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | methyl lactate + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | methyl mandelate + H2O | less than 1% activity compared to DL-pantoyl lactone | Fusarium proliferatum | ? | - |
? | |
3.1.1.25 | additional information | no activity with 4-nitrophenyl acetate and 4-nitrophenyl butyrate | Fusarium proliferatum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.25 | ? | x * 50000, recombinant protein, SDS-PAGE | Fusarium proliferatum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.25 | levo-lactonase | - |
Fusarium proliferatum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.1.1.25 | Fusarium proliferatum | by increasing the biomass concentration and lowering the inducer (isopropyl-beta-D-thiogalactoside) concentration and induction temperature, the recombinant lactonase production in Escherichia coli is significantly enhanced (20times higher than that of wild type strain Fusarium proliferatum ECU2002) | up |