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Literature summary extracted from

  • Torres-Rodriguez, B.I.; Flores-Berrout, K.; Villagomez-Castro, J.C.; Lopez-Romero, E.
    Purification and partial biochemical characterization of a membrane-bound type II-like alpha-glucosidase from the yeast morphotype of Sporothrix schenckii (2012), Antonie van Leeuwenhoek, 101, 313-322.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.207 1-deoxynojirimycin more specific inhibitor of alpha-glucosidase II than of alpha-glucosidase I Sporothrix schenckii
3.2.1.207 castanospermine
-
Sporothrix schenckii
3.2.1.207 additional information not inhibited by australine Sporothrix schenckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.207 0.0003
-
4-Methylumbelliferyl-alpha-D-glucopyranoside in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.207 membrane 50% of total enzyme activity in a mixed membrane fraction Sporothrix schenckii 16020
-
3.2.1.207 soluble 38% of total enzyme activity Sporothrix schenckii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.207 75400
-
2 * 75400, SDS-PAGE Sporothrix schenckii
3.2.1.207 152700
-
gel filtration Sporothrix schenckii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.207 Sporothrix schenckii
-
-
-
3.2.1.207 Sporothrix schenckii EH206
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.207 Mono Q column chromatography and Sephacryl S-300 gel filtration Sporothrix schenckii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.207 0.0535
-
soluble enzyme extract, using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii
3.2.1.207 0.457
-
after 8.5fold purification, using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii

Storage Stability

EC Number Storage Stability Organism
3.2.1.207 -20°C, purified enzyme, 4 days, 50% loss of activity Sporothrix schenckii
3.2.1.207 4°C, purified enzyme, 2 days, 50% loss of activity Sporothrix schenckii
3.2.1.207 4°C, soluble extract, 6 days, 50% loss of activity Sporothrix schenckii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.207 4-methylumbelliferyl-alpha-D-glucopyranoside + H2O high affinity Sporothrix schenckii 4-methylumbelliferol + alpha-D-glucopyranose
-
?
3.2.1.207 isomaltose + H2O digested at barely detectable level Sporothrix schenckii ?
-
?
3.2.1.207 isomaltose + H2O digested at barely detectable level Sporothrix schenckii EH206 ?
-
?
3.2.1.207 kojibiose + H2O
-
Sporothrix schenckii ?
-
?
3.2.1.207 kojibiose + H2O
-
Sporothrix schenckii EH206 ?
-
?
3.2.1.207 maltose + H2O
-
Sporothrix schenckii ?
-
?
3.2.1.207 maltose + H2O
-
Sporothrix schenckii EH206 ?
-
?
3.2.1.207 nigerose + H2O preferred substrate over kojibiose, trehalose, and isomaltose (clear preference of the enzyme for the alpha1,3 bond) Sporothrix schenckii ?
-
?
3.2.1.207 nigerose + H2O preferred substrate over kojibiose, trehalose, and isomaltose (clear preference of the enzyme for the alpha1,3 bond) Sporothrix schenckii EH206 ?
-
?
3.2.1.207 trehalose + H2O digested at barely detectable level Sporothrix schenckii ?
-
?
3.2.1.207 trehalose + H2O digested at barely detectable level Sporothrix schenckii EH206 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.207 homodimer 2 * 75400, SDS-PAGE Sporothrix schenckii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.207 alpha-glucosidase II
-
Sporothrix schenckii
3.2.1.207 type II-like alpha-glucosidase
-
Sporothrix schenckii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.207 37
-
-
Sporothrix schenckii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.207 7.2
-
-
Sporothrix schenckii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.207 0.00024
-
using nigerose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
3.2.1.207 0.00045
-
using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
3.2.1.207 0.00065
-
using kojibiose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
3.2.1.207 0.00074
-
using maltose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii 1-deoxynojirimycin
3.2.1.207 0.00145
-
using 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine
3.2.1.207 0.0032
-
using nigerose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine
3.2.1.207 0.004
-
using kojibiose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine
3.2.1.207 0.0068
-
using maltose as substrate, in 25 mM HEPES buffer, pH 7.2, at 37°C Sporothrix schenckii castanospermine