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Literature summary extracted from

  • Bruning, J.B.; Murillo, A.C.; Chacon, O.; Barletta, R.G.; Sacchettini, J.C.
    Structure of the Mycobacterium tuberculosis D-alanine:D-alanine ligase, a target of the antituberculosis drug D-cycloserine (2011), Antimicrob. Agents Chemother., 55, 291-301.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.4 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.4 t 2.1 A resoluion. Ddl is a dimer and consists of three discrete domains. The ligand binding cavity is at the intersection of all three domains and conjoined by several loop regions. The nucleotide and D-alanine binding pockets are flexible, requiring significant structural rearrangement of the bordering regions for entry and binding of both ATP and D-Ala molecules. D-cycloserine interacts with Ddl in a manner similar to that observed for D-Ala. Each ligand binds to two binding sites that have significant differences in affinity, with the first binding site exhibiting high affinity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.4 D-cycloserine preferential and weak inhibition at the second, lower-affinity binding site. D-cycloserine binding is tighter at higher ATP concentrations Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.4 Mycobacterium tuberculosis P9WP31
-
-
6.3.2.4 Mycobacterium tuberculosis H37Rv P9WP31
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.4 ATP + D-Ala + D-Ala
-
 Mycobacterium tuberculosis ADP + phosphate + D-Ala-D-Ala
-
 ?
6.3.2.4 ATP + D-Ala + D-Ala
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + D-Ala-D-Ala
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.2.4 0.37
-
 pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis D-cycloserine