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Literature summary extracted from

  • Iqbal, J.
    An enzyme immobilized microassay in capillary electrophoresis for characterization and inhibition studies of alkaline phosphatases (2011), Anal. Biochem., 414, 226-231.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.1 biotechnology a simple and fast dynamically coated capillary electrophoretic method is developed for the characterization and inhibition studies of alkaline phosphatases Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 arsenate irreversible inhibitor Bos taurus
3.1.3.1 phosphate competitive inhibitor Bos taurus
3.1.3.1 theophylline reversible non-competitive inhibitor Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.082
-
4-nitrophenyl phosphate pH 9.5, 37°C, Vmax: 0.96 nmol/min/mg enzyme, CIAP immobilized enzyme reactor (in excellent agreement with a Km value of calf ALP enzyme using a 96-well microplate reader) Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 intestine
-
Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
Bos taurus 4-nitrophenol + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
Bos taurus
3.1.3.1 calf intestinal alkaline phosphatase
-
Bos taurus
3.1.3.1 CIAP
-
Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 37
-
assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9.5
-
assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.1 0.000261
-
arsenate pH 9.5, 37°C, standard spectrophotometric assays Bos taurus
3.1.3.1 0.00241
-
phosphate pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay Bos taurus
3.1.3.1 0.00311
-
phosphate pH 9.5, 37°C, standard spectrophotometric assays Bos taurus
3.1.3.1 0.0089
-
arsenate pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay Bos taurus
3.1.3.1 0.05
-
theophylline pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay Bos taurus
3.1.3.1 0.096
-
theophylline pH 9.5, 37°C, standard spectrophotometric assays Bos taurus