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Literature summary extracted from

  • Gutka, H.J.; Franzblau, S.G.; Movahedzadeh, F.; Abad-Zapatero, C.
    Crystallization and preliminary X-ray characterization of the glpX-encoded class II fructose-1,6-bisphosphatase from Mycobacterium tuberculosis (2011), Acta Crystallogr. Sect. F, 67, 710-713.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.11 purified recombinant His-tagged enzyme, hanging-drop vapor-diffusion method, 0.001 ml of protein solution containing 10 mg/ml protein, is mixed with 0.001 ml of reservoir solution containing 1.8 M ammonium citrate tribasic, pH 7.0, and equilibratzion against 0.3 ml reservoir solution, 72 h to 2 weeks, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 37000
-
 x * 37000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Mycobacterium tuberculosis
-
 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Mycobacterium tuberculosis H37Rv
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Mycobacterium tuberculosis
-
 gene glpX
-
3.1.3.11 Mycobacterium tuberculosis H37Rv
-
 gene glpX
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Mycobacterium tuberculosis D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 ? x * 37000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 class II FBPase
-
 Mycobacterium tuberculosis
3.1.3.11 class II fructose-1,6-bisphosphatase
-
 Mycobacterium tuberculosis
3.1.3.11 FBPase
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.1.3.11 metabolism fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis Mycobacterium tuberculosis