Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hwang, H.; Kim, S.; Park, S.; Ngo, T.D.; Kim, K.K.; Kim, T.D.
    Crystallization and diffraction analysis of Sm23: an SGNH-family arylesterase from Sinorhizobium meliloti 1021 (2011), Acta Crystallogr. Sect. F, 67, 572-574.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.2 expressed in Escherichia coli XL1-Blue cells Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.2 microbatch method under oil, using 0.2 M magnesium formate as a precipitant Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 24000
-
 x * 24000, His-tagged enzyme, SDS-PAGE Sinorhizobium meliloti
3.1.1.2 24315
-
 x * 24315, His-tagged enzyme, calculated from amino acid sequence Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 ammonium sulfate precipitation, column chromatography, gel filtration Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 additional information the enzyme exhibits a substrate preference for short-chain fatty-acid esters such as C2 or C4 with an (R)-preference Sinorhizobium meliloti ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 ? x * 24000, His-tagged enzyme, SDS-PAGE Sinorhizobium meliloti
3.1.1.2 ? x * 24315, His-tagged enzyme, calculated from amino acid sequence Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
3.1.1.2 (R)-specific SGNH arylesterase
-
 Sinorhizobium meliloti
3.1.1.2 Sm23
-
 Sinorhizobium meliloti