Literature summary extracted from
Huang, K.; Ma, J.; Yuan, Y.; Gao, Y.
Cloning, expression, purification, crystallization and preliminary crystallographic analysis of NifH2 from Methanocaldococcus jannaschii (2011), Acta Crystallogr. Sect. F, 67, 133-135.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.18.6.1 |
gene MJ0685, expression of His-tagged NifH2 in Escherichia coli strain BL21 (DE3) |
Methanocaldococcus jannaschii |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.18.6.1 |
purified recombinant His-tagged NifH2, hanging drop vapour diffusion method, 0.001 l of 20 mg/ml protein in 2 mM Tris-HCl, pH 8.0, and 50 mM NaCl, is mixed with 0.001 ml of reservoir solution, containing 0.1 M sodium citrate, 8% PEG 8000, pH 5.0, and equilibrated against 0.15 ml of reservoir solution, 10 days, 4°C, X-ray diffraction structure determination and analysis at 2.85 A resolution |
Methanocaldococcus jannaschii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.18.6.1 |
Methanocaldococcus jannaschii |
- |
NifH2 is one of two homologues of NifH in Methanocaldococcus jannaschii |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.18.6.1 |
recombinant His-tagged NifH2 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration to over 95% purity |
Methanocaldococcus jannaschii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.18.6.1 |
NifH2 |
- |
Methanocaldococcus jannaschii |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.18.6.1 |
additional information |
nitrogenase is a protein complex that is required for biological nitrogen fixation. It is made up of a nitrogenase, which is a NifD2/NifK2 heterotetramer, and a nitrogenase reductase, which is a homodimer of NifH |
Methanocaldococcus jannaschii |