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Literature summary extracted from

  • Hoshino, T.; Nango, E.; Baba, S.; Eguchi, T.; Kumasaka, T.
    Crystallization and preliminary X-ray analysis of isopentenyl diphosphate isomerase from Methanocaldococcus jannaschii (2011), Acta Crystallogr. Sect. F, 67, 101-103.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expressed in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.2 sitting drop vapor diffusion method, using 0.6 M calcium acetate and 50 mM HEPES pH 7.5 Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 3,4-epoxy-3-methylbutyl diphosphate irreversible inhibition Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.3.2 Mg2+ IDI-2 requires a divalent cation such as Mg2+ for activity Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.2 40000
-
 x * 40000, SDS-PAGE Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.2 DEAE Sepharose column chromatography and Resource Phe column chromatograpyh Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
 Methanocaldococcus jannaschii dimethylallyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.3.2 ? x * 40000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
5.3.3.2 Idi-2
-
 Methanocaldococcus jannaschii
5.3.3.2 type 2 isopentenyl diphosphate isomerase
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.2 FMN required, IDI-2 reconstituted with cofactor analogues such as 5-deaza-FMN show no activity Methanocaldococcus jannaschii