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Literature summary extracted from

  • Anand, K.; Mathur, D.; Anant, A.; Garg, L.C.
    Structural studies of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv (2010), Acta Crystallogr. Sect. F, 66, 490-497.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.9 hanging drop vapor diffusion method, using 1.2 M ammonium sulfate pH 8.5 (0.1 M HEPES) and 5% (v/v) glycerol at 10°C Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.9 G157Y the mutation results in a complete loss of activity Mycobacterium tuberculosis
5.3.1.9 T211A the mutant shows decreased activity with higher Km compared with that of the wild type protein Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.9 Mycobacterium tuberculosis P9WN69
-
-
5.3.1.9 Mycobacterium tuberculosis H37Rv P9WN69
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.9 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.9 D-glucose 6-phosphate
-
 Mycobacterium tuberculosis D-fructose 6-phosphate
-
 ?
5.3.1.9 D-glucose 6-phosphate
-
 Mycobacterium tuberculosis H37Rv D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.9 homodimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
5.3.1.9 PGI
-
 Mycobacterium tuberculosis
5.3.1.9 Phosphoglucose isomerase
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
5.3.1.9 physiological function phosphoglucose isomerase plays a key role in both glycolysis and gluconeogenesis inside the cell, whereas outside the cell it exhibits cytokine properties. The enzyme also acts as an autocrine motility factor, a neuroleukin agent and a differentiation and maturation mediator Mycobacterium tuberculosis