BRENDA - Enzyme Database

The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4

Wilkinson, M.L.; Crary, S.M.; Jackman. J.E.; Grayhack, E.J.; Phizicky, E.M.; RNA 13, 404-413 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.225
expression in Escherichia coli
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.225
0.00001
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.225
S-adenosyl-L-methionine + adenosine4 in tRNAHis
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAPro
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.225
Saccharomyces cerevisiae
Q12383
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.225
-
Saccharomyces cerevisiae
Reaction
EC Number
Reaction
Commentary
Organism
2.1.1.225
S-adenosyl-L-methionine + adenosine4 in tRNAHis = S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
(3)
Saccharomyces cerevisiae
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
(2)
Saccharomyces cerevisiae
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAPro = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
(1)
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.225
S-adenosyl-L-methionine + adenosine4 in tRNAHis
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + adenosine4 in tRNAHis
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAPro
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAPro
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.225
30
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.225
0.004
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.225
8
-
assay at
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.225
expression in Escherichia coli
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.225
0.00001
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.225
S-adenosyl-L-methionine + adenosine4 in tRNAHis
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAPro
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.225
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.225
S-adenosyl-L-methionine + adenosine4 in tRNAHis
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + adenosine4 in tRNAHis
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAPro
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
2.1.1.225
S-adenosyl-L-methionine + cytidine4 in tRNAPro
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.225
30
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.225
0.004
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.225
8
-
assay at
Saccharomyces cerevisiae
General Information
EC Number
General Information
Commentary
Organism
2.1.1.225
malfunction
trm13-DELTA strains do not have an obvious growth defect
Saccharomyces cerevisiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.225
malfunction
trm13-DELTA strains do not have an obvious growth defect
Saccharomyces cerevisiae
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.1.1.225
400
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.1.1.225
400
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae