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Literature summary extracted from

  • Farkas, D.; Franzen, L.G.; Hansson, Ö.
    Cloning, expression and purification of the luminal domain of spinach photosystem 1 subunit PsaF functional in binding to plastocyanin and with a disulfide bridge required for folding (2011), Protein Expr. Purif., 78, 156-166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.97.1.12 cloning of the luminal domain of spinach photosystem 1 subunit PsaF, expressed in Escherichia coli BL21 (DE3) using a pET32 Xa/LIC thioredoxin fusion system Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.97.1.12 reduced plastocyanin + oxidized ferredoxin + hv Spinacia oleracea
-
oxidized plastocyanin + reduced ferredoxin
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.12 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.97.1.12 purification of the luminal domain of spinach photosystem 1 subunit PsaF Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.12 reduced plastocyanin + oxidized ferredoxin + hv
-
Spinacia oleracea oxidized plastocyanin + reduced ferredoxin
-
?
1.97.1.12 reduced plastocyanin + oxidized ferredoxin + hv the photosystem 1 subunit PsaF is involved in the docking of the electron-donor proteins plastocyanin and cytochrome c6, the recombinant protein binds to plastocyanin by a specific, native-like, electrostatic interaction Spinacia oleracea oxidized plastocyanin + reduced ferredoxin
-
?

Subunits

EC Number Subunits Comment Organism
1.97.1.12 ? 21000-22000 Da, photosystem 1 subunit PsaF that is involved in the docking of the electron-donor proteins plastocyanin and cytochrome c6, SDS-PAGE Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
1.97.1.12 PsaF
-
Spinacia oleracea