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Literature summary extracted from

  • Parsonage, D.; Desrosiers, D.C.; Hazlett, K.R.; Sun, Y.; Nelson, K.J.; Cox, D.L.; Radolf, J.D.; Poole, L.B.
    Broad specificity AhpC-like peroxiredoxin and its thioredoxin reductant in the sparse antioxidant defense system of Treponema pallidum (2010), Proc. Natl. Acad. Sci. USA, 107, 6240-6245.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.24 additional information TpAhpC is relatively resistant to inactivation during turnover with hydroperoxide substrates Treponema pallidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 0.0096
-
 cumene hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
1.11.1.24 0.012
-
 H2O2 pH 7.0, 25°C, TpAhpC Treponema pallidum
1.11.1.24 0.0388
-
 thioredoxin pH 7.0, 25°C, TpAhpC with cumene hydroperoxide Treponema pallidum
1.11.1.24 0.062
-
 thioredoxin pH 7.0, 25°C, TpAhpC with H2O2 Treponema pallidum
1.11.1.24 0.0794
-
 thioredoxin pH 7.0, 25°C, TpAhpC with t-butyl hydroperoxide Treponema pallidum
1.11.1.24 0.104
-
 t-butyl hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Treponema pallidum O83522 gene TP0509
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 2 thioredoxin + cumene hydroperoxide
-
 Treponema pallidum thioredoxin disulfide + H2O + 2-phenylpropan-2-ol
-
 ?
1.11.1.24 2 thioredoxin + H2O2
-
 Treponema pallidum thioredoxin disulfide + 2 H2O
-
 ?
1.11.1.24 2 thioredoxin + t-butyl hydroperoxide
-
 Treponema pallidum thioredoxin disulfide + H2O + t-butanol
-
 ?
1.11.1.24 additional information TpAhpC is a broad specificity peroxiredoxin, substrate specificity, overview Treponema pallidum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 More reduced TpAhpC forms stable octamers or decamers in solution whereas oxidation generates a heterogeneous mixture of oligomeric species. TpAhpC undergoes redox-sensitive oligomer formation Treponema pallidum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 AhpC-like peroxiredoxin
-
 Treponema pallidum
1.11.1.24 AhpC-like Prx
-
 Treponema pallidum
1.11.1.24 alkyl hydroperoxide reductase
-
 Treponema pallidum
1.11.1.24 More the enzyme belongs to the peroxiredoxin enzyme family Treponema pallidum
1.11.1.24 TP0509
-
 Treponema pallidum
1.11.1.24 typical 2-Cys Prx
-
 Treponema pallidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.24 25
-
 assay at Treponema pallidum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.24 9.8
-
 cumene hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
1.11.1.24 9.8
-
 thioredoxin pH 7.0, 25°C, TpAhpC with cumene hydroperoxide Treponema pallidum
1.11.1.24 17
-
 H2O2 pH 7.0, 25°C, TpAhpC Treponema pallidum
1.11.1.24 17
-
 thioredoxin pH 7.0, 25°C, TpAhpC with H2O2 Treponema pallidum
1.11.1.24 20.9
-
 t-butyl hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
1.11.1.24 20.9
-
 thioredoxin pH 7.0, 25°C, TpAhpC with t-butyl hydroperoxide Treponema pallidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.24 7
-
 assay at Treponema pallidum

General Information

EC Number General Information Comment Organism
1.11.1.24 additional information although this Tp peroxiredoxin closely resembles AhpC-like peroxidoxins, Treponema pallidum lacks AhpF, the typical reductant for such enzymes. Functionally, TpAhpC resembles largely eukaryotic, nonAhpC typical 2-Cys Prx proteins in using thioredoxin as an efficient electron donor and exhibiting broad specificity toward hydroperoxide substrates Treponema pallidum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.24 200
-
 t-butyl hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
1.11.1.24 250
-
 thioredoxin pH 7.0, 25°C, TpAhpC with cumene hydroperoxide Treponema pallidum
1.11.1.24 260
-
 thioredoxin pH 7.0, 25°C, TpAhpC with t-butyl hydroperoxide Treponema pallidum
1.11.1.24 270
-
 thioredoxin pH 7.0, 25°C, TpAhpC with H2O2 Treponema pallidum
1.11.1.24 1000
-
 cumene hydroperoxide pH 7.0, 25°C, TpAhpC Treponema pallidum
1.11.1.24 1400
-
 H2O2 pH 7.0, 25°C, TpAhpC Treponema pallidum