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Literature summary extracted from

  • Ali, T.; Ali, N.; Mohamed, L.
    Glutamine amidohydrolase from Penicillium politans NRC 510 (2009), Pol. J. Food Nutr. Sci., 59, 211-217.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 BaCl2 inhibition to a variable extent Penicillium palitans
3.5.1.2 CaCl2 inhibition to a variable extent Penicillium palitans
3.5.1.2 CuSO4 inhibition to a variable extent Penicillium palitans
3.5.1.2 HgCl2 inhibition to a variable extent Penicillium palitans
3.5.1.2 L-glutamate product inhibition, competitive Penicillium palitans
3.5.1.2 additional information no inhibition by 2-mercaptomethanol and iodoacetate Penicillium palitans
3.5.1.2 NaF inhibition to a variable extent Penicillium palitans
3.5.1.2 NH3 product inhibition Penicillium palitans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 additional information
-
 L-glutamate 7.5 mol/l, pH and temperature not specified in this publication Penicillium palitans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 NaCl 75% of the original activity in the presence of 15-30% NaCl Penicillium palitans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Penicillium palitans
-
 NRC 510
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 partially purified by heating and Sephadex G-100 gel filtration, 25% yield Penicillium palitans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 additional information
-
 133 U/mg Penicillium palitans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 D-asparagine + H2O
-
 Penicillium palitans D-aspartate + NH3
-
 ?
3.5.1.2 D-glutamine + H2O
-
 Penicillium palitans D-glutamate + NH3
-
 ?
3.5.1.2 L-asparagine + H2O
-
 Penicillium palitans L-aspartate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Penicillium palitans L-glutamate + NH3
-
 ?
3.5.1.2 additional information no hydrolysis of nicotinamide adenine dinucleotide, nicotinamide, and acetamide under the same experimental conditions as L-glutamine as substrate Penicillium palitans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 glutamine amidohydrolase
-
 Penicillium palitans
3.5.1.2 glutamine deamidating enzyme
-
 Penicillium palitans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 60
-
 purified enzyme Penicillium palitans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.2 60
-
 30 min at 60°C in the absence of the substrate has no effect on the activity of the partially purified enzyme Penicillium palitans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 7.5 8.5 purified enzyme Penicillium palitans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.2 additional information
-
 L-glutamate 39.0 mol/l, pH and temperature not specified in this publication Penicillium palitans