EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.3 | the bacterial luciferase lux gene cassette consists of five genes, luxCDABE. The lux operon is re-synthesized through a process of multibicistronic, codon-optimization to demonstrate self-directed bioluminescence emission in a mammalian HEK-293 cell line in vitro and in vivo, overview. To overcome the limitations by FMNH2 supply, co-expression of a constitutively expressed flavin reductase gene frp from Vibrio harveyi is performed leading to a 151fold increased increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes | Vibrio harveyi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.3 | additional information | codon optimization of the luxCDE and frp genes, e.g. adaptation of the bacterial protein to mammalian temperature of 37°C, detailed overview | Vibrio harveyi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | an aldehyde + FMNH2 + O2 | Vibrio harveyi | - |
a carboxylate + FMN + H2O + hnu | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.3 | Vibrio harveyi | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | an aldehyde + FMNH2 + O2 | - |
Vibrio harveyi | a carboxylate + FMN + H2O + hnu | - |
? | |
1.14.14.3 | an aldehyde + FMNH2 + O2 | a long chain aliphatic aldehyde as substrate | Vibrio harveyi | a carboxylate + FMN + H2O + hnu | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.3 | luciferase | - |
Vibrio harveyi |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.3 | physiological function | the luxCDABE encoded protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations | Vibrio harveyi |