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Literature summary extracted from

  • Rakwal R, Agrawal GK, Yonekura M, Kodama O.
    Naringenin 7-O-methyltransferase involved in the biosynthesis of the flavanone phytoalexin sakuranetin from rice (Oryza sativa L.) (2000), Plant Sci., 155, 213-221.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.232 after affinity chromatography, the enzyme becomes labile, losing almost all activity after 24 h storage at 4°C or at -20°C Oryza sativa
2.1.1.232 enzyme preparation after gel filtration retains more than 50% of its activity after 2 weeks at -20°C Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.232 Ba2+ 5 mM, 60% inhibition Oryza sativa
2.1.1.232 Ca2+ 5 mM, 63% inhibition Oryza sativa
2.1.1.232 Cd2+ 5 mM, complete inhibition Oryza sativa
2.1.1.232 Co2+ 5 mM, 39% inhibition Oryza sativa
2.1.1.232 Cu2+ 5 mM, complete inhibition Oryza sativa
2.1.1.232 EDTA 10 mM, 31% inhibition Oryza sativa
2.1.1.232 Hg2+ 5 mM, complete inhibition Oryza sativa
2.1.1.232 Mg2+ 5 mM, 40% inhibition Oryza sativa
2.1.1.232 Mn2+ 5 mM, complete inhibition Oryza sativa
2.1.1.232 Ni2+ 5 mM, complete inhibition Oryza sativa
2.1.1.232 Zn2+ 5 mM, complete inhibition Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.232 38000
-
 gel filtration Oryza sativa
2.1.1.232 41000
-
 1 * 41000, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.232 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone Oryza sativa i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone Oryza sativa L. Hitomebore i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.232 Oryza sativa
-
-
-
2.1.1.232 Oryza sativa L. Hitomebore
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.232
-
 Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.232 leaf UV-irradiated Oryza sativa
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.232 -20°C, enzyme in crude homogenates is stable for over 1 month Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.232 additional information no activity with 3,5,7,4'-tetrahydroxyflavanone (kaempferol), 5,7,4'-trihydroxyisoflavone (genistein), 7,4'-dihydroxyisoflavone (daidzein), 5,4'-dihydroxy-7-methoxyflavanone (sakuranetin), or 3,4-dihydroxycinnamic acid (caffeic acid) Oryza sativa ?
-
 ?
2.1.1.232 additional information no activity with 3,5,7,4'-tetrahydroxyflavanone (kaempferol), 5,7,4'-trihydroxyisoflavone (genistein), 7,4'-dihydroxyisoflavone (daidzein), 5,4'-dihydroxy-7-methoxyflavanone (sakuranetin), or 3,4-dihydroxycinnamic acid (caffeic acid) Oryza sativa L. Hitomebore ?
-
 ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins Oryza sativa S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin Oryza sativa S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin. The enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins Oryza sativa L. Hitomebore S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin Oryza sativa L. Hitomebore S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin ?
2.1.1.232 S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone i.e. luteolin, 148% of the activity with naringenin Oryza sativa S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
-
 ?
2.1.1.232 S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone i.e. luteolin, 148% of the activity with naringenin Oryza sativa L. Hitomebore S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
-
 ?
2.1.1.232 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin, 81% of the activity with naringenin Oryza sativa S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?
2.1.1.232 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin, 81% of the activity with naringenin Oryza sativa L. Hitomebore S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?

Subunits

EC Number Subunits Comment Organism
2.1.1.232 monomer 1 * 41000, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.232 NOMT
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.232 7.5 9 Tris-HCl buffer Oryza sativa
2.1.1.232 8 10 glycine-NaOH buffer Oryza sativa

Expression

EC Number Organism Comment Expression
2.1.1.232 Oryza sativa the enzyme is induced by UV irradiation, jasmonic acid and CuCl2 (weak) up

General Information

EC Number General Information Comment Organism
2.1.1.232 physiological function the enzyme is involved in the biosynthesis of the flavanone phytoalexin sakuranetin. Plants possess inducible defence mechanisms against pathogen attack that include the production of phytoalexins. The product of the reaction, sakuranetin is a major rice phytoalexin accumulating both in ultraviolet irradiated and blast infected as well as in copper chloride or jasmonic acid, treated rice leaves Oryza sativa